Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/97639
Title: The magnitude of dengue virus NS1 protein secretion is strain dependent and does not correlate with severe pathologies in the mouse infection model
Authors: Watanabe, Satoru
Tan, Kah Hin
Rathore, Abhay P. S.
Rozen-Gagnon, Kathryn
Shuai, Wang
Ruedl, Christiane
Vasudevan, Subhash G.
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Watanabe, S., Tan, K. H., Rathore, A. P. S., Rozen-Gagnon, K., Shuai, W., Ruedl, C., et al. (2012). The magnitude of dengue virus NS1 protein secretion is strain dependent and does not correlate with severe pathologies in the mouse infection model. Journal of Virology, 86(10), 5508-5514.
Series/Report no.: Journal of virology
Abstract: There are conflicting data on the relationship between the level of secreted NS1 (sNS1), viremia, and disease severity upon dengue virus (DENV) infection in the clinical setting, and therefore, we examined this relationship in the widely accepted AG129 mouse model. Because of the failure of a routinely used NS1 detection kit to detect sNS1 of the mouse-adapted DENV2 strain, we screened 15 previously undescribed NS1 monoclonal antibodies and developed a robust capture enzyme-linked immunosorbent assay (ELISA) with detection sensitivity at the low nanogram level (0.2 ng/ml) using recombinant baculovirus-expressed sNS1 as well as sNS1 that was immunoaffinity purified from the various DENV2 strains employed in this study. Using this test, we demonstrated that increased viremia paralleled severe pathologies; however, sNS1 level did not correlate with viremia or severity. Furthermore, among the DENV2 strains that were tested, the level of NS1 secretion did not correspond to virus replication rate in vitro, at the cellular level. Together, our data indicate that the magnitude of NS1 secretion appears to be strain dependent and does not correlate with viral virulence in the AG129 mouse model.
URI: https://hdl.handle.net/10356/97639
http://hdl.handle.net/10220/11196
DOI: 10.1128/JVI.07081-11
Schools: School of Biological Sciences 
Rights: © 2012 American Society of Microbiology.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

SCOPUSTM   
Citations 10

51
Updated on Mar 17, 2024

Web of ScienceTM
Citations 5

52
Updated on Oct 25, 2023

Page view(s) 10

757
Updated on Mar 15, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.