Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/104425
Title: | [Retracted] Peroxisome proliferator-activated receptor β/δ induces myogenesis by modulating myostatin activity | Authors: | Bonala, Sabeera Lokireddy, Sudarsanareddy Arigela, Harikumar Teng, Serena Wahli, Walter Sharma, Mridula McFarlane, Craig Kambadur, Ravi |
Keywords: | DRNTU::Science::Biological sciences::Biochemistry | Issue Date: | 2012 | Source: | Bonala, S., Lokireddy, S., Arigela, H., Teng, S., Wahli, W., Sharma, M., et al. (2012). Peroxisome proliferator-activated receptor β/δ induces myogenesis by modulating myostatin activity. Journal of biological chemistry, 287(16), 12935-12951. [Retracted] | Series/Report no.: | Journal of biological chemistry | Abstract: | Classically, peroxisome proliferator-activated receptor β/δ (PPARβ/δ) function was thought to be restricted to enhancing adipocyte differentiation and development of adipose-like cells from other lineages. However, recent studies have revealed a critical role for PPARβ/δ during skeletal muscle growth and regeneration. Although PPARβ/δ has been implicated in regulating myogenesis, little is presently known about the role and, for that matter, the mechanism(s) of action of PPARβ/δ in regulating postnatal myogenesis. Here we report for the first time, using a PPARβ/δ-specific ligand (L165041) and the PPARβ/δ-null mouse model, that PPARβ/δ enhances postnatal myogenesis through increasing both myoblast proliferation and differentiation. In addition, we have identified Gasp-1 (growth and differentiation factor-associated serum protein-1) as a novel downstream target of PPARβ/δ in skeletal muscle. In agreement, reduced Gasp-1 expression was detected in PPARβ/δ-null mice muscle tissue. We further report that a functional PPAR-responsive element within the 1.5-kb proximal Gasp-1 promoter region is critical for PPARβ/δ regulation of Gasp-1. Gasp-1 has been reported to bind to and inhibit the activity of myostatin; consistent with this, we found that enhanced secretion of Gasp-1, increased Gasp-1 myostatin interaction and significantly reduced myostatin activity upon L165041-mediated activation of PPARβ/δ. Moreover, we analyzed the ability of hGASP-1 to regulate myogenesis independently of PPARβ/δ activation. The results revealed that hGASP-1 protein treatment enhances myoblast proliferation and differentiation, whereas silencing of hGASP-1 results in defective myogenesis. Taken together these data revealed that PPARβ/δ is a positive regulator of skeletal muscle myogenesis, which functions through negatively modulating myostatin activity via a mechanism involving Gasp-1. | URI: | https://hdl.handle.net/10356/104425 http://hdl.handle.net/10220/17069 |
DOI: | 10.1074/jbc.M111.319145 | Schools: | School of Biological Sciences | Rights: | © 2012 by The American Society for Biochemistry and Molecular Biology, Inc | Fulltext Permission: | none | Fulltext Availability: | No Fulltext |
Appears in Collections: | SBS Journal Articles |
SCOPUSTM
Citations
20
27
Updated on Mar 16, 2024
Web of ScienceTM
Citations
10
27
Updated on Oct 31, 2023
Page view(s) 10
892
Updated on Mar 18, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.