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https://hdl.handle.net/10356/79319
Title: | An essential role for maternal control of Nodal signaling | Authors: | Tran, Long Duc Kumari, Pooja Gilligan, Patrick C. Lim, Shimin Winkler, Sylke Philp, Robin Sampath, Karuna |
Keywords: | DRNTU::Science::Biological sciences | Issue Date: | 2013 | Source: | Kumari, P., Gilligan, P. C., Lim, S., Tran, L. D., Winkler, S., Philp, R., et al. (2013). An essential role for maternal control of Nodal signaling. eLife, 2, e00683-. | Series/Report no.: | eLife | Abstract: | Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor, Squint (Sqt), are localized to future embryonic dorsal. The mechanisms by which maternal sqt/nodal RNA is localized and regulated have been unclear. Here, we show that maternal control of Nodal signaling via the conserved Y box-binding protein 1 (Ybx1) is essential. We identified Ybx1 via a proteomic screen. Ybx1 recognizes the 3’ untranslated region (UTR) of sqt RNA and prevents premature translation and Sqt/Nodal signaling. Maternal-effect mutations in zebrafish ybx1 lead to deregulated Nodal signaling, gastrulation failure, and embryonic lethality. Implanted Nodal-coated beads phenocopy ybx1 mutant defects. Thus, Ybx1 prevents ectopic Nodal activity, revealing a new paradigm in the regulation of Nodal signaling, which is likely to be conserved. | URI: | https://hdl.handle.net/10356/79319 http://hdl.handle.net/10220/17445 |
ISSN: | 2050-084X | DOI: | 10.7554/eLife.00683 | Schools: | School of Biological Sciences | Rights: | © 2013 Kumari et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
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An essential role for maternal control of Nodal signaling.pdf | 4.77 MB | Adobe PDF | View/Open |
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