Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/79319
Title: An essential role for maternal control of Nodal signaling
Authors: Tran, Long Duc
Kumari, Pooja
Gilligan, Patrick C.
Lim, Shimin
Winkler, Sylke
Philp, Robin
Sampath, Karuna
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2013
Source: Kumari, P., Gilligan, P. C., Lim, S., Tran, L. D., Winkler, S., Philp, R., et al. (2013). An essential role for maternal control of Nodal signaling. eLife, 2, e00683-.
Series/Report no.: eLife
Abstract: Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor, Squint (Sqt), are localized to future embryonic dorsal. The mechanisms by which maternal sqt/nodal RNA is localized and regulated have been unclear. Here, we show that maternal control of Nodal signaling via the conserved Y box-binding protein 1 (Ybx1) is essential. We identified Ybx1 via a proteomic screen. Ybx1 recognizes the 3’ untranslated region (UTR) of sqt RNA and prevents premature translation and Sqt/Nodal signaling. Maternal-effect mutations in zebrafish ybx1 lead to deregulated Nodal signaling, gastrulation failure, and embryonic lethality. Implanted Nodal-coated beads phenocopy ybx1 mutant defects. Thus, Ybx1 prevents ectopic Nodal activity, revealing a new paradigm in the regulation of Nodal signaling, which is likely to be conserved.
URI: https://hdl.handle.net/10356/79319
http://hdl.handle.net/10220/17445
ISSN: 2050-084X
DOI: 10.7554/eLife.00683
Schools: School of Biological Sciences 
Rights: © 2013 Kumari et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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