Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/99893
Title: | Focal targeting by human β-defensin 2 disrupts localized virulence factor assembly sites in Enterococcus faecalis | Authors: | Normark, Staffan Kandaswamy, Kumaravel Liew, Tze Horng Huston-Warren, Emily Meyer-Hoffert, Ulf Hultenby, Kjell Henriques-Normark, Birgitta Wang, Charles Y. Schröder, Jens M. Caparon, Michael G. Hultgren, Scott J. Kline, Kimberly A. |
Keywords: | DRNTU::Science::Biological sciences::Microbiology::Bacteria | Issue Date: | 2013 | Source: | Kandaswamy, K., Liew, T. H., Wang, C. Y., Huston-Warren, E., Meyer-Hoffert, U., Hultenby, K., et al. (2013). Focal targeting by human β-defensin 2 disrupts localized virulence factor assembly sites in Enterococcus faecalis. Proceedings of the National Academy of Sciences of the United States of America, 110(50), 20230-20235. | Series/Report no.: | Proceedings of the National Academy of Sciences of the United States of America | Abstract: | Virulence factor secretion and assembly occurs at spatially restricted foci in some Gram-positive bacteria. Given the essentiality of the general secretion pathway in bacteria and the contribution of virulence factors to disease progression, the foci that coordinate these processes are attractive antimicrobial targets. In this study, we show in Enterococcus faecalis that SecA and Sortase A, required for the attachment of virulence factors to the cell wall, localize to discrete domains near the septum or nascent septal site as the bacteria proceed through the cell cycle. We also demonstrate that cationic human β-defensins interact with E. faecalis at discrete septal foci, and this exposure disrupts sites of localized secretion and sorting. Modification of anionic lipids by multiple peptide resistance factor, a protein that confers antimicrobial peptide resistance by electrostatic repulsion, renders E. faecalis more resistant to killing by defensins and less susceptible to focal targeting by the cationic antimicrobial peptides. These data suggest a paradigm in which focal targeting by antimicrobial peptides is linked to their killing efficiency and to disruption of virulence factor assembly. | URI: | https://hdl.handle.net/10356/99893 http://hdl.handle.net/10220/18581 |
DOI: | 10.1073/pnas.1319066110 | Schools: | School of Biological Sciences | Organisations: | Singapore Centre for Environmental Life Sciences Engineering | Rights: | © 2013 The Authors. This paper was published in Proceedings of the National Academy of Sciences of the United States of America and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.1073/pnas.1319066110]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles SCELSE Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Focal targeting by human β-defensin 2 disrupts localized virulence factor assembly sites in Enterococcus faecalis.pdf | 1.17 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
5
61
Updated on Mar 16, 2024
Web of ScienceTM
Citations
10
49
Updated on Oct 24, 2023
Page view(s) 10
835
Updated on Mar 18, 2024
Download(s) 10
343
Updated on Mar 18, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.