Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/106197
Title: | Bactericidal mechanisms revealed for rapid water disinfection by superabsorbent cryogels decorated with silver nanoparticles | Authors: | Fane, Anthony Gordon Loo, Siew-Leng Krantz, William B. Gao, Yiben Lim, Teik-Thye Hu, Xiao |
Keywords: | DRNTU::Science::Biological sciences::Microbiology::Bacteria | Issue Date: | 2015 | Source: | Loo, S. L., Krantz, W. B., Fane, A. G., Gao, Y., Lim, T.-T., & Hu, X. (2015). Bactericidal mechanisms revealed for rapid water disinfection by superabsorbent cryogels decorated with silver nanoparticles. Environmental science & technology, 49(4), 2310-2318. | Series/Report no.: | Environmental science & technology | Abstract: | The authors have recently reported the fabrication of superabsorbent cryogels decorated with silver nanoparticles (PSA/AgNP cryogels) that demonstrate rapid water disinfection. This paper provides a systematic elucidation of the bactericidal mechanisms of AgNPs (silver nanoparticles), both generally and in the specific context of cryogels. Direct contact between the PSA/AgNP cryogel interface and the bacterial cells is required to accomplish disinfection. Specifically, the disinfection efficacy is closely correlated to the cell-bound Ag concentration, which constitutes >90% of the Ag released. Cells exposed to PSA/AgNP cryogels show a significant depletion of intracellular adenosine triphosphate (ATP) content and cell-membrane lesions. A positive ROS (reactive oxygen species) scavenging test confirms the involvement of ROS (·O2–, H2O2, and ·OH) in the bactericidal mechanism. Furthermore, exposed bacterial cells show an enhanced level of thiobarbituric acid reactive substances, indicating the occurrence of cell-membrane peroxidation mediated by ROS. In addition, this study reveals that both Ag+ and Ag0 are involved in the bactericidal mechanism of AgNPs via tests conducted using PSA cryogels with bound Ag+ ions (or PSA/Ag+ cryogels without reducing Ag+ to Ag0). Significantly, bacterial cells exposed to PSA/Ag+ cryogels did not show any cell-membrane damage even though the former had a higher cell-bound Ag concentration than that of the PSA/AgNP cryogels, thus indicating the differential action of Ag+ and Ag0. | URI: | https://hdl.handle.net/10356/106197 http://hdl.handle.net/10220/34459 |
ISSN: | 0013-936X | DOI: | 10.1021/es5048667 | Schools: | School of Civil and Environmental Engineering School of Materials Science & Engineering |
Research Centres: | Nanyang Environment and Water Research Institute | Rights: | © 2015 American Chemical Society (ACS). This is the author created version of a work that has been peer reviewed and accepted for publication by Environmental Science & Technology, American Chemical Society (ACS). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/es5048667]. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | CEE Journal Articles MSE Journal Articles NEWRI Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Clean manuscript - es-2014-048667 (R1) - 3.pdf | Main article | 1.05 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
5
81
Updated on Mar 22, 2024
Web of ScienceTM
Citations
5
67
Updated on Oct 31, 2023
Page view(s) 20
699
Updated on Mar 29, 2024
Download(s) 20
321
Updated on Mar 29, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.