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Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells

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Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells

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dc.contributor.author Kang, Tae Bong
dc.contributor.author Yoo, Yung Choon
dc.contributor.author Lee, Kwan Hee
dc.contributor.author Yoon, Ho Sup
dc.contributor.author Her, Erk
dc.contributor.author Kim, Jong Bae
dc.contributor.author Song, Seong Kyu
dc.date.accessioned 2011-05-09T07:31:14Z
dc.date.available 2011-05-09T07:31:14Z
dc.date.copyright 2008
dc.date.issued 2011-05-09T07:31:14Z
dc.identifier.citation Kang, T. B., Yoo, Y. C., Lee, K. H., Yoon, H. S., Her, E., Kim, J. B., et al. (2008). Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide(NO) production in murine macrophage cells. Journal of biomedical science, 15(2).
dc.identifier.uri http://hdl.handle.net/10220/6780
dc.description.abstract Synthesis of nitric oxide (NO) is one of the important effector functions of innate immune cells. Although several reports have indicated mistletoe lectins induce immune cells to produce cytokines, studies regarding the activities of the lectins in the production of NO have been very limited. Here, we report on the induction of NO synthesis in a murine macrophage cell line, RAW264.7, by Korean mistletoe lectin (KML-IIU). When the macrophage cells were treated with KML-IIU in the presence of a suboptimal concentration of IFN-γ, NO production was induced in a concentration-dependent manner. Significantly higher levels of NO were induced by subchains of the KML-IIU (A and B), which have lower toxicities, as compared to the hololectin. Furthermore, expression of the inducible nitric oxide synthase (iNOS) gene was elevated in accordance with the level of NO production. When the synthase was inhibited by iNOS inhibitors (L-NIL and L-NAME), NO production was specifically reduced in a concentration-dependent manner. Our studies demonstrate that the KML-IIU and its subchains induce NO production in murine macrophage cells via activation of the iNOS gene expression, suggesting that the KML-IIU subchains may be used as an immunomodulator to enhance the effector functions of innate immune cells.
dc.format.extent 19 p.
dc.language.iso en
dc.relation.ispartofseries Journal of biomedical science
dc.rights © 2008 Springer Verlag This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biomedical Science, Springer Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1007/s11373-007-9210-2].
dc.subject DRNTU::Science::Biological sciences::Microbiology::Immunology.
dc.title Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1007/s11373-007-9210-2
dc.description.version Accepted version

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