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Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors.

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Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors.

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dc.contributor.author Madhavan Nallani.
dc.contributor.author Hans-Peter M. de Hoog.
dc.contributor.author Jeroen J. L. M. Cornelissen.
dc.contributor.author Alan E. Rowan.
dc.contributor.author Roeland J. M. Nolte.
dc.contributor.author Isabel W.C.E.Arends.
dc.date.accessioned 2011-05-11T03:23:10Z
dc.date.available 2011-05-11T03:23:10Z
dc.date.copyright 2009
dc.date.issued 2011-05-11T03:23:10Z
dc.identifier.citation Madhavan, N., Hans-Peter, M. H., Jeroen, J. L. M. C., Alan, E. R., Roeland, J. M. N., & Isabel, W. C. E. A. (2009). Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors. Organic and biomolecular chemistry, 7, 4604-4610.
dc.identifier.issn 1477-0520
dc.identifier.uri http://hdl.handle.net/10220/6788
dc.description.abstract The encapsulation of chloroperoxidase from Caldariomyces fumago (CPO) in block copolymer polymersomes is reported. Fluorescence and electron microscopy show that when the encapsulating conditions favour self-assembly of the block copolymer, the enzyme is incorporated with concentrations that are 50 times higher than the enzyme concentration before encapsulation. The oxidation of two substrates by the encapsulated enzyme was studied: i) pyrogallol, a common substrate used to assay CPO enzymatic activity and ii) thioanisole, of which the product, (R)-methyl phenyl sulfoxide, is an important pharmaceutical intermediate. The CPO-loaded polymersomes showed distinct reactivity towards these substrates.While the oxidation of pyrogallol was limited by diffusion of the substrate into the polymersome, the rate-limiting step for the oxidation of thioansiole was the turnover by the enzyme.
dc.format.extent 7 p.
dc.language.iso en
dc.relation.ispartofseries Organic and biomolecular chemistry
dc.subject DRNTU::Engineering::Materials::Biomaterials.
dc.title Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors.
dc.type Journal Article
dc.contributor.school School of Materials Science and Engineering
dc.identifier.doi http://dx.doi.org/10.1039/b911370c
dc.description.version Published version
dc.identifier.rims 155295

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