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CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex

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CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex

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dc.contributor.author Choi, Yuk Kwan
dc.contributor.author Liu, Pengfei
dc.contributor.author Sze, Siu Kwan
dc.contributor.author Dai, Chao
dc.contributor.author Qi, Robert Z.
dc.date.accessioned 2011-05-25T02:40:26Z
dc.date.available 2011-05-25T02:40:26Z
dc.date.copyright 2010
dc.date.issued 2011-05-25T02:40:26Z
dc.identifier.citation Choi, Y. K., Liu, P., Sze, S. K., Dai, C., & Qi, R. Z. (2010). CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex. Journal of cell biology, 191(6), 1089-1095.
dc.identifier.uri http://hdl.handle.net/10220/6796
dc.description.abstract CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the γ-tubulin ring complex (γ-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the γ-TuRC–mediated nucleation activator (γ-TuNA). γ-TuNA but not its γ-TuC–binding-deficient mutant stimulates microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. γ-TuRC bound to γ-TuNA contains NME7, FAM128A/B, and actin in addition to γ-tubulin and GCP2–6. RNA interference–mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although γ-TuRC assembly is unaffected. Collectively, these results suggest that the γ-TuNA found in CDK5RAP2 has regulatory functions in γ-TuRC–mediated microtubule nucleation.
dc.language.iso en
dc.relation.ispartofseries Journal of cell biology
dc.rights © 2010 The Rockefeller University Press. This paper was published in Journal of Cell Biology and is made available as an electronic reprint (preprint) with permission of The Rockefeller University Press. The paper can be found at the following DOI:http://dx.doi.org/10.1083/jcb.201007030. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
dc.subject DRNTU::Science::Biological sciences::Molecular biology.
dc.title CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1083/jcb.201007030
dc.description.version Published version

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