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A hydrogel-based enzyme-loaded polymersome reactor

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A hydrogel-based enzyme-loaded polymersome reactor

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dc.contributor.author De Hoog, Hans-Peter M.
dc.contributor.author Arends, Isabel W. C. E.
dc.contributor.author Rowan, Alan E.
dc.contributor.author Cornelissen, Jeroen J. L. M.
dc.contributor.author Nolte, Roeland J. M.
dc.date.accessioned 2011-09-05T03:31:38Z
dc.date.available 2011-09-05T03:31:38Z
dc.date.copyright 2010
dc.date.issued 2011-09-05
dc.identifier.citation De Hoog, H. P. M., Arends, I. W. C. E., Rowan, A. E., Cornelissen, J. J. L. M., & Nolte, R. J. M. (2010). A hydrogel-based enzyme-loaded polymersome reactor. Nanoscale, 2, 709-716.
dc.identifier.issn 2040-3364
dc.identifier.uri http://hdl.handle.net/10220/6970
dc.description.abstract In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarcticalipase B (CALB) and glucose oxidase (GOx).
dc.format.extent 8 p.
dc.language.iso en
dc.relation.ispartofseries Nanoscale
dc.rights © 2010 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Nanoscale, The Royal Society of Chemistry.  It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: [DOI: http://dx.doi.org/10.1039/b9nr00325h].
dc.subject DRNTU::Engineering::Materials::Biomaterials
dc.title A hydrogel-based enzyme-loaded polymersome reactor
dc.type Journal Article
dc.contributor.school School of Materials Science and Engineering
dc.identifier.doi http://dx.doi.org/10.1039/b9nr00325h
dc.description.version Accepted version
dc.identifier.rims 155296

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