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Membrane-based electrochemical nanobiosensor for escherichia coli detection and analysis of cells viability

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Membrane-based electrochemical nanobiosensor for escherichia coli detection and analysis of cells viability

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dc.contributor.author Cheng, Ming Soon
dc.contributor.author Lau, Suk Hiang
dc.contributor.author Chow, Vincent T. K.
dc.contributor.author Toh, Chee-Seng
dc.date.accessioned 2011-09-14T08:58:32Z
dc.date.available 2011-09-14T08:58:32Z
dc.date.copyright 2011
dc.date.issued 2011-09-14
dc.identifier.citation Cheng, M. S., Lau, S. H., Chow, V. T. K., & Toh, C. S. (2011). Membrane-based electrochemical nanobiosensor for escherichia coli detection and analysis of cells viability. Environmental science & technology, 45(15), 6453-6459.
dc.identifier.issn 0013-936X
dc.identifier.uri http://hdl.handle.net/10220/7054
dc.description.abstract A sensitive and selective membrane-based electrochemical nanobiosensor is developed for specific quantitative label-free detection of Escherichia coli (E. coli) cells and analysis of viable but nonculturable (VBNC) E. coli cells which remain mostly undetected using current methods. The sensing mechanism relies on the blocking of nanochannels of a nanoporous alumina-membrane modified electrode, upon the formation of immune complexes at the nanoporous membrane. The resulting obstacle to diffusive mass transfer of a redox probe in the analysis solution to the underlying platinum electrode reduces the Faradaic signal response of the biosensor, measured using cyclic voltammetry. Antibody loading under conditions of varying antibody concentrations and pHs are optimized. The biosensor gives a low detection limit of 22 cfu mL ̄¹ (R² = 0.999) over a wide linear working range of 10 to 10⁶cfu mL ̄¹. It is specific toward E. coli with minimal cross-reactivity to two other pathogenic bacteria (commonly found in waters). Relative standard deviation (RSD) for triplicate measurements of 2.5% indicates reasonably useful level of reproducibility. Differentiation of live, VBNC, and dead cells are carried out after the cell capture and quantitation step, by simple monitoring of the cells’ enzyme activity using the same redox probe in the analysis solution, in the presence of glucose.
dc.format.extent 7 p.
dc.language.iso en
dc.relation.ispartofseries Environmental science & technology
dc.rights © 2011 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Environmental Science & Technology, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at:[DOI: http://dx.doi.org/10.1021/es200884a ]
dc.subject DRNTU::Science::Chemistry::Biochemistry::Water analysis.
dc.title Membrane-based electrochemical nanobiosensor for escherichia coli detection and analysis of cells viability
dc.type Journal Article
dc.contributor.school School of Physical and Mathematical Sciences
dc.identifier.doi http://dx.doi.org/10.1021/es200884a
dc.description.version Accepted version
dc.identifier.rims 161435

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