mirage

Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly.

DSpace/Manakin Repository

 

Search DR-NTU


Advanced Search Subject Search

Browse

My Account

Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly.

Show simple item record

dc.contributor.author Lu, Lei.
dc.contributor.author Ladinsky, Mark S.
dc.contributor.author Kirchhausen, Tomas.
dc.date.accessioned 2011-10-05T06:58:11Z
dc.date.available 2011-10-05T06:58:11Z
dc.date.copyright 2011
dc.date.issued 2011-10-05
dc.identifier.citation Lu, L., Ladinsky, M. S., & Kirchhausen, T. (2011). Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly. The Journal of Cell Biology, 194(3), 425-440.
dc.identifier.uri http://hdl.handle.net/10220/7161
dc.description.abstract During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope after their formation. In the prepore model, nucleoporins assemble on the chromatin as an intermediate nuclear pore complex before nuclear envelope formation. Using live-cell imaging and electron microscope tomography, we find that the mitotic assembly of the nuclear envelope primarily originates from ER cisternae. Moreover, the nuclear pore complexes assemble only on the already formed nuclear envelope. Indeed, all the chromatin-associated Nup107–160 complexes are in single units instead of assembled prepores. We therefore propose that the postmitotic nuclear envelope assembles directly from ER cisternae followed by membrane-dependent insertion of nuclear pore complexes.
dc.language.iso en
dc.relation.ispartofseries The journal of cell Biology
dc.rights © 2011 Lu et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
dc.subject DRNTU::Science::Biological sciences::Molecular biology.
dc.title Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly.
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1083/jcb.201012063
dc.description.version Published version

Files in this item

Files Size Format View
Formation of th ... ssembly (published v.).pdf 5.044Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Statistics

Total views

All Items Views
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly. 370

Total downloads

All Bitstreams Views
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly (published v.).pdf 190

Top country downloads

Country Code Views
United States of America 59
China 58
Singapore 36
Russian Federation 9
Taiwan 5

Top city downloads

city Views
Mountain View 56
Singapore 34
Shanghai 7
Taipei 5
Zaporozhye 2