Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription.

DSpace/Manakin Repository


Search DR-NTU

Advanced Search Subject Search


My Account

Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription.

Show simple item record

dc.contributor.author Tan, Jen Jen.
dc.contributor.author Ong, Angeline Shufen.
dc.contributor.author Chen, Ken-Shiung.
dc.date.accessioned 2011-10-06T03:09:20Z
dc.date.available 2011-10-06T03:09:20Z
dc.date.copyright 2011
dc.date.issued 2011-10-06
dc.identifier.citation Tan, J. J., Ong, S. F., & Chen, K.-S. (2011). Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. BMC Molecular Biology, 12(4).
dc.identifier.issn 1471-2199
dc.identifier.uri http://hdl.handle.net/10220/7166
dc.description.abstract The Rasd1 protein is a dexamethasone induced monomeric Ras-like G protein that oscillates in the suprachiasmatic nucleus (SCN). Previous studies have shown that Rasd1 modulates multiple signaling cascades. However, it is still unclear exactly how Rasd1 carries out its function. Studying protein-protein interactions involving Rasd1 may provide insights into its biological functions in different contexts. Results: To further explore the molecular function of Rasd1, we performed a yeast two-hybrid screen and identified Ear2, a negative regulator of renin transcription, as an interaction partner of Rasd1. We validated the interaction in vitro and in transfected COS-7 cells. We further confirmed the interaction of endogenous Rasd1 and Ear2 from HEK293T cell and mouse brain extract. Rasd1 inhibited transcriptional repression by Ear2 on a renin promoter-luciferase reporter construct both in the presence and absence of all-trans-retinoic acid. Moreover, realtime RT-PCR showed upregulation of endogenous renin transcription in As4.1 cells over-expressing Rasd1. We demonstrated that the ligand binding domain of Ear2 is required for physical and functional interaction between the two proteins. In addition, we demonstrated that shRNA-mediated knockdown of Rasd1 results in further repression of Ear2-mediated renin transcription, whereas induction of Rasd1 by dexamethasone counteracts the effects of shRNA-mediated Rasd1 knockdown. Finally, our study showed that Rasd1 missense mutations not only attenuate their physical interaction with Ear2 but also abolish their ability to counteract repression of renin transcription mediated by Ear2. Conclusions: Our study provides evidence for physical and functional interactions between Rasd1 and Ear2. The results suggest that their interactions are involved in renin transcriptional regulation. These findings not only reveal a novel role for Rasd1-medated signaling but also provide the basis for potential intervention of renin expression.
dc.format.extent 17 p.
dc.language.iso en
dc.relation.ispartofseries BMC molecular biology
dc.rights Copyright 2011 The Authors. The journal's website is located at http://www.biomedcentral.com/bmcmolbiol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subject DRNTU::Science::Biological sciences::Molecular biology.
dc.title Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription.
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1186/1471-2199-12-4
dc.description.version Published version

Files in this item

Files Size Format View
Rasd1 Ear2.pdf 2.057Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record


Total views

All Items Views
Rasd1 interacts with Ear2 (Nr2f6) to regulate renin transcription. 417

Total downloads

All Bitstreams Views
Rasd1 Ear2.pdf 253

Top country downloads

Country Code Views
China 84
United States of America 65
Singapore 41
Taiwan 20
Russian Federation 11

Top city downloads

city Views
Mountain View 49
Beijing 48
Singapore 40
Tainan 10
Southampton 2

Downloads / month

  2015-02 2015-03 2015-04 total
Rasd1 Ear2.pdf 0 0 6 6