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Title:
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Rasd1 modulates the coactivator function of NonO in the cyclic AMP pathway.
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Author:
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Ong, Angeline Shufen.; Tan, Jen Jen.; Tew, Wai Loon.; Chen, Ken Shiung.
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Copyright year:
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2011 |
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Abstract:
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All living organisms exhibit autonomous daily physiological and behavioural rhythms to help
them synchronize with the environment. Entrainment of circadian rhythm is achieved via activation of
cyclic AMP (cAMP) and mitogen-activated protein kinase signaling pathways. NonO (p54nrb) is a
multifunctional protein involved in transcriptional activation of the cAMP pathway and is involved in
circadian rhythm comtrol. Rasd1 is a monomeric G protein implicated to play a pivotal role in
potentiating both photic and nonphotic responses of the circadian rhythm. In this study, we have
identified and validated NonO as an interacting partner of Rasd1 via affinity pulldown, coimmunoprecipitation
and indirect immunofluorescence studies. The GTP-hydrolysis activity of Rasd1
is required for the functional interaction. Functional interaction of Rasd1-NonO in the cAMP pathway
was investigated via reporter gene assays, chromatin immunoprecipitation and gene knockdown. We
showed that Rasd1 and NonO interact at the CRE-site of specific target genes. These findings reveal a
novel mechanism by which the coregulator activity of NonO can be modulated. |
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Subject:
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DRNTU::Science::Biological sciences::Molecular biology. |
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Type:
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Journal Article |
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Series/ Journal Title:
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PLoS ONE |
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School:
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School of Biological Sciences |
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Rights:
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Copyright 2011 The Authors. The journal's website is located at http://www.plosone.org. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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Version:
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Published version |
