mirage

NMDA receptor activation enhances inhibitory GABAergic transmission onto hippocampal pyramidal neurons via presynaptic and postsynaptic mechanisms

DSpace/Manakin Repository

 

Search DR-NTU


Advanced Search Subject Search

Browse

My Account

NMDA receptor activation enhances inhibitory GABAergic transmission onto hippocampal pyramidal neurons via presynaptic and postsynaptic mechanisms

Show simple item record

dc.contributor.author Xue, Jiu Gang
dc.contributor.author Masuoka, Takayoshi
dc.contributor.author Gong, Xian Di
dc.contributor.author Chen, Ken-Shiung
dc.contributor.author Yanagawa, Yuchio
dc.contributor.author Law, Alex Sai Kit
dc.contributor.author Konishi, Shiro
dc.date.accessioned 2011-10-13T04:23:12Z
dc.date.available 2011-10-13T04:23:12Z
dc.date.copyright 2011
dc.date.issued 2011-10-13
dc.identifier.citation Xue, J. G., Masuoka, T., Gong, X. D., Chen, K.-S., Yanagawa, Y., Law, A. S. K., & Konishi, S. (2011). NMDA Receptor Activation Enhances Inhibitory GABAergic Transmission onto Hippocampal Pyramidal Neurons via Presynaptic and Postsynaptic Mechanisms. Journal of Neurophysiology, 105(6), 2897-2906.
dc.identifier.issn 0022-3077
dc.identifier.uri http://hdl.handle.net/10220/7260
dc.description.abstract N-methyl-D-aspartate (NMDA) receptors (NMDARs) are implicated in synaptic plasticity and modulation of glutamatergic excitatory transmission. Effect of NMDAR activation on inhibitory GABAergic transmission remains largely unknown. Here, we report that a brief application of NMDA could induce two distinct actions in CA1 pyramidal neurons in mouse hippocampal slices: 1) an inward current attributed to activation of postsynaptic NMDARs; and 2) fast phasic synaptic currents, namely spontaneous inhibitory postsynaptic currents (sIPSCs), mediated by GABAA receptors in pyramidal neurons. The mean amplitude of sIPSCs was also increased by NMDA. This profound increase in the sIPSC frequency and amplitude was markedly suppressed by the sodium channel blocker TTX, whereas the frequency and mean amplitude of miniature IPSCs were not significantly affected by NMDA, suggesting that NMDA elicits repetitive firing in GABAergic interneurons, thereby leading to GABA release from multiple synaptic sites of single GABAergic axons. We found that the NMDAR openchannel blocker MK-801 injected into recorded pyramidal neurons suppressed the NMDA-induced increase of sIPSCs, which raises the possibility that the firing of interneurons may not be the sole factor and certain retrograde messengers may also be involved in the NMDA-mediated enhancement of GABAergic transmission. Our results from pharmacological tests suggest that the nitric oxide signaling pathway is mobilized by NMDAR activation in CA1 pyramidal neurons, which in turn retrogradely facilitates GABA release from the presynaptic terminals. Thus NMDARs at glutamatergic synapses on both CA1 pyramidal neurons and interneurons appear to exert feedback and feedforward inhibition for determining the spike timing of the hippocampal microcircuit.
dc.format.extent 37 p.
dc.language.iso en
dc.relation.ispartofseries Journal of neurophysiology
dc.rights © 2011 the American Physiological Society.
dc.subject DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology.
dc.title NMDA receptor activation enhances inhibitory GABAergic transmission onto hippocampal pyramidal neurons via presynaptic and postsynaptic mechanisms
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1152/jn.00287.2010

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Statistics

Total views

All Items Views
NMDA receptor activation enhances inhibitory GABAergic transmission onto hippocampal pyramidal neurons via presynaptic and postsynaptic mechanisms 413

Total downloads

All Bitstreams Views
J Neurophysiol manuscript.pdf 6

Top country downloads

Country Code Views
Singapore 4

Top city downloads

city Views
Singapore 4