In vitro studies of magnetically enhanced transfection in COS-7 cells

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In vitro studies of magnetically enhanced transfection in COS-7 cells

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dc.contributor.author Ang, Derrick
dc.contributor.author Tay, Chor Yong
dc.contributor.author Tan, L. P.
dc.contributor.author Preiser, Peter Rainer
dc.contributor.author Ramanujan, Raju V.
dc.date.accessioned 2011-10-13T08:04:51Z
dc.date.available 2011-10-13T08:04:51Z
dc.date.copyright 2011
dc.date.issued 2011-10-13
dc.identifier.citation Ang, D., Tay, C. Y., Tan, L. P., Preiser, P. R., & Ramanujan, R. V. (2011). In vitro studies of magnetically enhanced transfection in COS-7 cells. Materials science and engineering : C, 31, 1455-1457.
dc.identifier.uri http://hdl.handle.net/10220/7267
dc.description.abstract In the magnetically enhanced gene delivery technique, DNA complexed with polymer coated aggregated magnetic nanoparticles (AMNPs) is used for effecting transfection. The aim of this study is to examine the relationship between transfection efficiency and the physical characteristics of the polymer coated AMNPs. In vitro studies of transfection efficiency in COS-7 cells were carried out using pEGFP-N1 and pMIR-REPORT complexed polyethylenimine (PEI) coated iron oxide magnetic nanoparticles. PEI coated AMNPs (PEI-AMNPs) with average individual particle diameters in the range of 8 nm to 30 nm were studied and characterized by transmission electron microscopy, vibrating sample magnetometry, X-ray diffractometry, thermal gravimetric analysis and photon correlation spectroscopy methods. PEI-A8MNP and PEI-A30MNP yielded higher transfection efficiency compared to commercial polyMAG particles as well as PEI of equivalent molar ratio of nitrogen/phosphorous (N/P ratio). The transfection efficiency was related to the physical characteristics of the PEI-AMNPs and its complexes: transfection efficiency was strongly positively correlated with saturation magnetization (Ms) and susceptibility (χ), strongly negatively correlated with N/P ratio, moderately positively correlated to zeta potential and moderately negatively correlated to hydrodynamic diameter of the complex. PEI-A8MNP and PEI-A30MNP possessing higher Ms, χ, lower N/P ratio and smaller complex size exhibited higher transfection efficiency compared to PEI-A16MNP which have weaker magnetic properties, higher N/P ratio and larger complex size.
dc.format.extent 13 p.
dc.language en
dc.relation.ispartofseries Materials science and engineering : C
dc.rights © 2011 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Materials science and engineering: C, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1016/j.msec.2011.05.014 ]
dc.subject DRNTU::Engineering::Materials::Magnetic materials.
dc.title In vitro studies of magnetically enhanced transfection in COS-7 cells
dc.type Journal Article
dc.identifier.doi http://dx.doi.org/10.1016/j.msec.2011.05.014
dc.description.version Accepted version
dc.identifier.rims 161870

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