Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/94360
Title: In vitro studies of magnetically enhanced transfection in COS-7 cells
Authors: Ang, Derrick
Tay, Chor Yong
Tan, L. P.
Ramanujan, Raju V.
Preiser, Peter Rainer
Keywords: DRNTU::Engineering::Materials::Magnetic materials
Issue Date: 2011
Source: Ang, D., Tay, C. Y., Tan, L. P., Preiser, P. R., & Ramanujan, R. V. (2011). In vitro studies of magnetically enhanced transfection in COS-7 cells. Materials science and engineering : C, 31, 1455-1457.
Series/Report no.: Materials science and engineering : C
Abstract: In the magnetically enhanced gene delivery technique, DNA complexed with polymer coated aggregated magnetic nanoparticles (AMNPs) is used for effecting transfection. The aim of this study is to examine the relationship between transfection efficiency and the physical characteristics of the polymer coated AMNPs. In vitro studies of transfection efficiency in COS-7 cells were carried out using pEGFP-N1 and pMIR-REPORT complexed polyethylenimine (PEI) coated iron oxide magnetic nanoparticles. PEI coated AMNPs (PEI-AMNPs) with average individual particle diameters in the range of 8 nm to 30 nm were studied and characterized by transmission electron microscopy, vibrating sample magnetometry, X-ray diffractometry, thermal gravimetric analysis and photon correlation spectroscopy methods. PEI-A8MNP and PEI-A30MNP yielded higher transfection efficiency compared to commercial polyMAG particles as well as PEI of equivalent molar ratio of nitrogen/phosphorous (N/P ratio). The transfection efficiency was related to the physical characteristics of the PEI-AMNPs and its complexes: transfection efficiency was strongly positively correlated with saturation magnetization (Ms) and susceptibility (χ), strongly negatively correlated with N/P ratio, moderately positively correlated to zeta potential and moderately negatively correlated to hydrodynamic diameter of the complex. PEI-A8MNP and PEI-A30MNP possessing higher Ms, χ, lower N/P ratio and smaller complex size exhibited higher transfection efficiency compared to PEI-A16MNP which have weaker magnetic properties, higher N/P ratio and larger complex size.
URI: https://hdl.handle.net/10356/94360
http://hdl.handle.net/10220/7267
DOI: 10.1016/j.msec.2011.05.014
Rights: © 2011 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Materials science and engineering: C, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1016/j.msec.2011.05.014 ]
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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