mirage

Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.

DSpace/Manakin Repository

 

Search DR-NTU


Advanced Search Subject Search

Browse

My Account

Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.

Show simple item record

dc.contributor.author Agarwal, Kan.
dc.contributor.author Baek, Kwanghee.
dc.contributor.author Jeon, Choon Ju.
dc.contributor.author Miyamoto, Kenichi.
dc.contributor.author Ueno, Akemichi.
dc.contributor.author Yoon, Ho Sup.
dc.date.accessioned 2012-01-26T03:05:36Z
dc.date.available 2012-01-26T03:05:36Z
dc.date.copyright 1991
dc.date.issued 2012-01-26
dc.identifier.citation Agarwal, K., Baek, K., Jeon, C., Miyamoto, K., Ueno, A., & Yoon, H. (1991). Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS. Biochemistry, 30(31), 7842-7851.
dc.identifier.uri http://hdl.handle.net/10220/7480
dc.description.abstract The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in conjunction with the polymerase binding region for transcriptional stimulation. Interestingly, a mutant TFIIS with only the polymerase binding domain actually inhibits transcription, whereas a mutant in which the polymerase binding and zinc finger domains are separated by an octapeptide is only weakly active. The zinc finger itself has no effect on transcription, but in contrast to the wild-type protein, it binds to oligonucleotides. These findings suggest that TFIIS may interact with RNA polymerase II such that the normally masked zinc finger can specifically contact nucleotides in the transcription elongation zone at a position juxtaposed to the polymerization site.
dc.language.iso en
dc.relation.ispartofseries Biochemistry
dc.rights © 1991 American Chemical Society.
dc.subject DRNTU::Science::Chemistry::Biochemistry.
dc.title Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1021/bi00245a026

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record