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Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506

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Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506

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dc.contributor.author Kotaka, Masayo
dc.contributor.author Ye, Hong
dc.contributor.author Alag, Reema
dc.contributor.author Hu, Guangan
dc.contributor.author Bozdech, Zbynek
dc.contributor.author Preiser, Peter Rainer
dc.contributor.author Yoon, Ho Sup
dc.contributor.author Lescar, Julien
dc.date.accessioned 2012-01-26T03:26:27Z
dc.date.available 2012-01-26T03:26:27Z
dc.date.copyright 2008
dc.date.issued 2012-01-26
dc.identifier.citation Kotaka, M., Ye, H., Alag, R., Hu, G., Bozdech, Z., Preiser, P. R., et al. (2008). Crystal Structure of the FK506 Binding Domain of Plasmodium falciparum FKBP35 in Complex with FK506. Biochemistry, 47(22), 5951-5961.
dc.identifier.uri http://hdl.handle.net/10220/7482
dc.description.abstract The emergence of multi-drug-resistant strains of Plasmodium parasites has prompted the search for alternative therapeutic strategies for combating malaria. One possible strategy is to exploit existing drugs as lead compounds. FK506 is currently used in the clinic for preventing transplant rejection. It binds to a α/β protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. We report here the crystallographic structure at 2.35 Å resolution of PfFKBD complexed with FK506. Compared to the human FKBP12−FK506 complex reported earlier, the structure reveals structural differences in the β5−β6 segment that lines the FK506 binding site. The presence in PfFKBD of Cys-106 and Ser-109 (substituting for His-87 and Ile-90, respectively, in human FKBP12), which are 4−5 Å from the nearest atom of the FK506 compound, suggests possible routes for the rational design of analogues of FK506 with specific antiparasitic activity. Upon ligand binding, several conformational changes occur in PfFKBD, including aromatic residues that shape the FK506 binding pocket as shown by NMR studies. A microarray analysis suggests that FK506 and cyclosporine A (CsA) might inhibit parasite development by interfering with the same signaling pathways.
dc.language.iso en
dc.relation.ispartofseries Biochemistry
dc.rights © 2008 American Chemical Society.
dc.subject DRNTU::Science::Biological sciences::Microbiology::Virology
dc.title Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1021/bi800004u

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