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The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II.

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The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II.

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dc.contributor.author Jeon, Choon Ju.
dc.contributor.author Yoon, Ho Sup.
dc.contributor.author Agarwal, Kan.
dc.date.accessioned 2012-01-26T08:17:57Z
dc.date.available 2012-01-26T08:17:57Z
dc.date.copyright 1994
dc.date.issued 2012-01-26
dc.identifier.citation Jeon, C. J., Yoon, H. S. & Agarwal, K. (1994). The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II. Proceedings of the National Academy of Sciences, pp.9106–9110.
dc.identifier.uri http://hdl.handle.net/10220/7484
dc.description.abstract The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. Substitution of either of these residues inactivates both TFIIS functions, suggesting a related role in both activities. These acidic residues may participate in phosphoryl transfer reactions by a two-metal-ion mechanism in a manner analogous to Klenow fragment. The RNA polymerase II itself may contain a Zn ribbon, in as much as the polymerase's 15-kDa subunit contains a sequence that aligns well with the TFIIS Zn ribbon sequence, including a similarly placed pair of acidic residues.
dc.language.iso en
dc.rights © 1994 National Academy of Sciences.  This is the author created version of a work that has been peer reviewed and accepted for publication by Proceedings of the National Academy of Sciences , National Academy of Sciences.  It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: http://dx.doi.org/10.1073/pnas.91.19.9106
dc.subject DRNTU::Science::Chemistry::Biochemistry.
dc.title The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II.
dc.type Conference Paper
dc.contributor.conference National Academy of Sciences (1994)
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1073/pnas.91.19.9106
dc.description.version Accepted version

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