mirage

The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II

DSpace/Manakin Repository

 

Search DR-NTU


Advanced Search Subject Search

Browse

My Account

The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II

Show simple item record

dc.contributor.author Jeon, Choon Ju
dc.contributor.author Yoon, Ho Sup
dc.contributor.author Agarwal, Kan
dc.date.accessioned 2012-01-26T08:17:57Z
dc.date.available 2012-01-26T08:17:57Z
dc.date.copyright 1994
dc.date.issued 2012-01-26
dc.identifier.citation Jeon, C. J., Yoon, H. S. & Agarwal, K. (1994). The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II. Proceedings of the National Academy of Sciences, pp.9106–9110.
dc.identifier.uri http://hdl.handle.net/10220/7484
dc.description.abstract The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. Substitution of either of these residues inactivates both TFIIS functions, suggesting a related role in both activities. These acidic residues may participate in phosphoryl transfer reactions by a two-metal-ion mechanism in a manner analogous to Klenow fragment. The RNA polymerase II itself may contain a Zn ribbon, in as much as the polymerase's 15-kDa subunit contains a sequence that aligns well with the TFIIS Zn ribbon sequence, including a similarly placed pair of acidic residues.
dc.language.iso en
dc.rights © 1994 National Academy of Sciences.  This is the author created version of a work that has been peer reviewed and accepted for publication by Proceedings of the National Academy of Sciences , National Academy of Sciences.  It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: http://dx.doi.org/10.1073/pnas.91.19.9106
dc.subject DRNTU::Science::Chemistry::Biochemistry.
dc.title The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II
dc.type Conference Paper
dc.contributor.conference National Academy of Sciences (1994)
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1073/pnas.91.19.9106
dc.description.version Accepted version

Files in this item

Files Size Format View
45. The transcr ... bbon dipeptide Asp-Glu.pdf 738.9Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Statistics

Total views

All Items Views
The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II 618

Total downloads

All Bitstreams Views
45. The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu.pdf 624

Top country downloads

Country Code Views
China 345
Singapore 128
United States of America 87
Taiwan 16
Ukraine 10

Top city downloads

city Views
Beijing 172
Singapore 128
Mountain View 51
Nanchang 40
Shenyang 40

Downloads / month

  2014-10 2014-11 2014-12 total
45. The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu.pdf 0 0 11 11