mirage

Determination of encephalomyocarditis viral diabetogenicity by a putative binding site of the viral capsid protein

DSpace/Manakin Repository

 

Search DR-NTU


Advanced Search Subject Search

Browse

My Account

Determination of encephalomyocarditis viral diabetogenicity by a putative binding site of the viral capsid protein

Show full item record

Title: Determination of encephalomyocarditis viral diabetogenicity by a putative binding site of the viral capsid protein
Author: Jun, Hee-Sook; Kang, Yup; Yoon, Ho Sup; Kim, Ki Hwan; Notkins, Abner L.; Yoon, Ji-Won
Copyright year: 1998
Abstract: The molecular mechanism by which some, but not all, variants of encephalomyocarditis (EMC) virus selectively infect pancreatic beta-cells in mice and induce IDDM has been an enigma for more than a decade. We report here that the binding site of the EMC viral capsid protein VP1 determines viral diabetogenicity. Recombinant chimeric EMC viruses containing threonine, serine, proline, aspartic acid, or valine at position 152 of the major capsid protein VP1 bind poorly to beta-cells. In contrast, recombinant chimeric EMC viruses containing alanine or glycine at position 152 of the VP1 bind efficiently to and infect beta-cells, resulting in the development of diabetes. Three-dimensional molecular modeling reveals that the van der Waals interactions are greater and the residues surrounding position 152 of the VP1 are more closely packed in recombinant chimeric viruses containing threonine, serine, proline, aspartic acid, or valine at position 152 than in recombinant chimeric viruses containing alanine or glycine at the same position. Our studies reveal that the surface areas surrounding alanine or glycine at position 152 of the VP1 are more accessible, thus increasing the availability of the binding sites for attachment to beta-cell receptors and resulting in viral infection and the development of diabetes.
Subject: DRNTU::Science::Biological sciences::Microbiology::Virology.
Type: Journal Article
Series/ Journal Title: Diabetes
School: School of Biological Sciences
Rights: © 1998 American Diabetes Association. This is the author created version of a work that has been peer reviewed and accepted for publication by Diabetes, American Diabetes Association. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.2337/diabetes.47.4.576
Version: Accepted version

Files in this item

Files Size Format View
36. Determinati ... ral Diabetogenicity by.pdf 508.4Kb PDF View/Open
   

DOI Query

- Get published version (via Digital Object Identifier)
   

This item appears in the following Collection(s)

Show full item record

Statistics

Total views

All Items Views
Determination of encephalomyocarditis viral diabetogenicity by a putative binding site of the viral capsid protein 354

Total downloads

All Bitstreams Views
36. Determination of Encephalomyocarditis Viral Diabetogenicity by.pdf 210

Top country downloads

Country Code Views
China 71
United States of America 57
Singapore 34
Germany 5
Indonesia 5

Top city downloads

city Views
Beijing 52
Mountain View 39
Singapore 34
Redwood City 5
Shenzhen 5

Downloads / month

  2014-07 2014-08 2014-09 total
36. Determination of Encephalomyocarditis Viral Diabetogenicity by.pdf 0 0 2 2