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Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy.

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Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy.

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dc.contributor.author Zhang, Enming.
dc.contributor.author Xue, Renhao.
dc.contributor.author Soo, Jianchow.
dc.contributor.author Chen, Peng.
dc.date.accessioned 2012-02-14T07:13:45Z
dc.date.available 2012-02-14T07:13:45Z
dc.date.copyright 2008
dc.date.issued 2012-02-14
dc.identifier.citation Zhang, E., Xue, R., Soo, J. & Chen, P. (2008). Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy. Pflügers Archiv European Journal of Physiology, 457, 211-222.
dc.identifier.uri http://hdl.handle.net/10220/7522
dc.description.abstract Exocytosis of neurotransmitter or hormone-filled vesicles is a highly dynamic process regulated by various proteins and lipids. As mainly revealed indirectly by the electrophysiological methods, exocytosis is believed to involve multiple kinetic steps in which vesicles transit from one state to another. Using total internal reflection fluorescence microscopy which enables direct visualization of individual vesicles, we developed an analytical framework to track and analyze vesicle dynamics. We demonstrated that all subplasmalemmal vesicles generally undergo constant and caged Brownian motion. And they can be classified into three populations that differ in their motion characteristics and fusion competence. Furthermore, we showed that these vesicle pools are differentially modulated by phorbol-12-myristate-13-acetate, a phorbol ester analog to endogenous diacylglycerol, through both protein-kinase- C-dependent and -independent pathways.
dc.language.iso en
dc.relation.ispartofseries Pflügers archiv European journal of physiology
dc.rights © 2008 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Pflügers Archiv European Journal of Physiology, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1007/s00424-008-0485-6 ]
dc.subject DRNTU::Science::Medicine::Biomedical engineering.
dc.title Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy.
dc.type Journal Article
dc.contributor.school School of Chemical and Biomedical Engineering
dc.identifier.doi http://dx.doi.org/10.1007/s00424-008-0485-6
dc.description.version Accepted version

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