| dc.contributor.author |
Zhang, Enming. |
| dc.contributor.author |
Xue, Renhao. |
| dc.contributor.author |
Soo, Jianchow. |
| dc.contributor.author |
Chen, Peng. |
| dc.date.accessioned |
2012-02-14T07:13:45Z |
| dc.date.available |
2012-02-14T07:13:45Z |
| dc.date.copyright |
2008 |
| dc.date.issued |
2012-02-14 |
| dc.identifier.citation |
Zhang, E., Xue, R., Soo, J. & Chen, P. (2008). Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy. Pflügers Archiv European Journal of Physiology, 457, 211-222. |
| dc.identifier.uri |
http://hdl.handle.net/10220/7522 |
| dc.description.abstract |
Exocytosis of neurotransmitter or hormone-filled
vesicles is a highly dynamic process regulated by various
proteins and lipids. As mainly revealed indirectly by the
electrophysiological methods, exocytosis is believed to
involve multiple kinetic steps in which vesicles transit from
one state to another. Using total internal reflection
fluorescence microscopy which enables direct visualization
of individual vesicles, we developed an analytical framework
to track and analyze vesicle dynamics. We demonstrated
that all subplasmalemmal vesicles generally undergo
constant and caged Brownian motion. And they can be
classified into three populations that differ in their motion
characteristics and fusion competence. Furthermore, we
showed that these vesicle pools are differentially modulated
by phorbol-12-myristate-13-acetate, a phorbol ester analog
to endogenous diacylglycerol, through both protein-kinase-
C-dependent and -independent pathways. |
| dc.language.iso |
en |
| dc.relation.ispartofseries |
Pflügers archiv European journal of physiology |
| dc.rights |
© 2008 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Pflügers Archiv European Journal of Physiology, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1007/s00424-008-0485-6 ] |
| dc.subject |
DRNTU::Science::Medicine::Biomedical engineering. |
| dc.title |
Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy. |
| dc.type |
Journal Article |
| dc.contributor.school |
School of Chemical and Biomedical Engineering |
| dc.identifier.doi |
http://dx.doi.org/10.1007/s00424-008-0485-6 |
| dc.description.version |
Accepted version |
