Solution structure of a pleckstrin-homology domain

DSpace/Manakin Repository


Search DR-NTU

Advanced Search Subject Search


My Account

Solution structure of a pleckstrin-homology domain

Show simple item record

dc.contributor.author Yoon, Ho Sup
dc.contributor.author Hajduk, Philip J.
dc.contributor.author Petros, Andrew M.
dc.contributor.author Olejniczak, Edward T.
dc.contributor.author Meadows, Robert P.
dc.contributor.author Fesik, Stephen W.
dc.date.accessioned 2012-03-22T02:52:23Z
dc.date.available 2012-03-22T02:52:23Z
dc.date.copyright 1994
dc.date.issued 2012-03-22
dc.identifier.citation Yoon, H. S., Hajduk, P. J., Petros, A. M., Olejniczak, E. T., Meadows, R. P., & Fesik, S. W. (1994). Solution structure of a pleckstrin-homology domain. Nature, 369, 672-675.
dc.identifier.uri http://hdl.handle.net/10220/7656
dc.description.abstract PLECKSTRIN1, the major protein kinase C substrate of platelets, contains domains of about 100 amino acids at the amino and carboxy termini that have been found in a number of proteins, including serine/threonine kinases, GTPase-activating proteins, phospholipases and cytoskeletal proteins2–5. These conserved sequences, termed pleckstrin-homology (PH) domains, are thought to be involved in signal transduction. But the details of the function and binding partners of the PH domains have not been characterized. Here we report the solution structure of the N-terminal pleckstrin-homology domain of pleckstrin determined using heteronuclear three-dimensional nuclear magnetic resonance spectroscopy. The structure consists of an up-and-down β-barrel of seven antiparallel β-strands and a C-terminal amphiphilic α-helix that caps one end of the barrel. The overall topology of the domain is similar to that of the retinol-binding protein family of structures6–10.
dc.format.extent 10 p.
dc.language.iso en
dc.relation.ispartofseries Nature
dc.rights © 1994 Nature Publishing Group. This is the author created version of a work that has been peer reviewed and accepted for publication by Nature, Nature Publishing Group. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at:http://dx.doi.org/10.1038/369672a0
dc.subject DRNTU::Science::Biological sciences
dc.title Solution structure of a pleckstrin-homology domain
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1038/369672a0
dc.description.version Accepted version

Files in this item

Files Size Format View
46. Solution st ... kstrin-homology domain.pdf 488.9Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record


Total views

All Items Views
Solution structure of a pleckstrin-homology domain 592

Total downloads

All Bitstreams Views
46. Solution structure of a pleckstrin-homology domain.pdf 272

Top country downloads

Country Code Views
United States of America 118
China 57
Singapore 36
France 10
Algeria 6

Top city downloads

city Views
Mountain View 72
Singapore 35
Beijing 10
Chengdu 5
Redwood City 5