Microfluidic platform for controlling the differentiation of embryoid bodies

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Microfluidic platform for controlling the differentiation of embryoid bodies

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Title: Microfluidic platform for controlling the differentiation of embryoid bodies
Author: Fung, Wai-To; Beyzavi, Ali; Abgrall, Patrick; Nguyen, Nam-Trung; Li, Hoi-Yeung
Copyright year: 2009
Abstract: Embryonic stem (ES) cells are pluripotent cells, which can differentiate into any cell type. This cell type has often been implicated as an eminent source of renewable cells for tissue regeneration and cellular replacement therapies. Studies on manipulation of the various differentiation pathways have been at the forefront of research. There are many ways in which ES cells can be differentiated. One of the most common techniques is to initiate the development of embryoid bodies (EBs) by in vitro aggregation of ES cells. Thereafter, EBs can be induced to undergo differentiation into various cell lineages. In this article, we present a microfluidic platform using biocompatible materials, which is suitable for culturing EBs. The platform is based on a Y-channel device with two inlets for two different culturing media. An EB is located across both streams. Using the laminar characteristics at low Reynolds number and high Peclet numbers, we have induced cell differentiation on half of the EB while maintaining the other half in un-induced stages. The results prove the potential of using microfluidic technology for manipulation of EBs and ES cells in tissue engineering.
Subject: DRNTU::Engineering::Mechanical engineering
Type: Journal Article
Series/ Journal Title: Lab on a chip
School: School of Mechanical and Aerospace Engineering
Rights: © 2009 Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Lab on a Chip, Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: DOI: [http://dx.doi.org/10.1039/B903753E].
Version: Accepted version

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