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Microfluidic platform for controlling the differentiation of embryoid bodies

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Microfluidic platform for controlling the differentiation of embryoid bodies

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dc.contributor.author Fung, Wai-To
dc.contributor.author Beyzavi, Ali
dc.contributor.author Abgrall, Patrick
dc.contributor.author Nguyen, Nam-Trung
dc.contributor.author Li, Hoi-Yeung
dc.date.accessioned 2012-04-12T05:54:42Z
dc.date.available 2012-04-12T05:54:42Z
dc.date.copyright 2009
dc.date.issued 2012-04-12
dc.identifier.citation Fung, W. T., Beyzavi, A., Abgrall, P., Nguyen, N. T., & Li, H..Y. (2009). Microfluidic platform for controlling the differentiation of embryoid body. Lab on a Chip, 9(17), 2591–2595.
dc.identifier.uri http://hdl.handle.net/10220/7751
dc.description.abstract Embryonic stem (ES) cells are pluripotent cells, which can differentiate into any cell type. This cell type has often been implicated as an eminent source of renewable cells for tissue regeneration and cellular replacement therapies. Studies on manipulation of the various differentiation pathways have been at the forefront of research. There are many ways in which ES cells can be differentiated. One of the most common techniques is to initiate the development of embryoid bodies (EBs) by in vitro aggregation of ES cells. Thereafter, EBs can be induced to undergo differentiation into various cell lineages. In this article, we present a microfluidic platform using biocompatible materials, which is suitable for culturing EBs. The platform is based on a Y-channel device with two inlets for two different culturing media. An EB is located across both streams. Using the laminar characteristics at low Reynolds number and high Peclet numbers, we have induced cell differentiation on half of the EB while maintaining the other half in un-induced stages. The results prove the potential of using microfluidic technology for manipulation of EBs and ES cells in tissue engineering.
dc.format.extent 6 p.
dc.language.iso en
dc.relation.ispartofseries Lab on a chip
dc.rights © 2009 Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Lab on a Chip, Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: DOI: [http://dx.doi.org/10.1039/B903753E].
dc.subject DRNTU::Engineering::Mechanical engineering
dc.title Microfluidic platform for controlling the differentiation of embryoid bodies
dc.type Journal Article
dc.contributor.school School of Mechanical and Aerospace Engineering
dc.identifier.doi http://dx.doi.org/10.1039/B903753E
dc.description.version Accepted version

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