Enhanced electrophoretic DNA separation in photonic crystal fiber

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Enhanced electrophoretic DNA separation in photonic crystal fiber

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Title: Enhanced electrophoretic DNA separation in photonic crystal fiber
Author: Sun, Yi; Nguyen, Nam-Trung; Kwok, Yien Chian
Copyright year: 2009
Abstract: Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip.
Subject: DRNTU::Engineering::Mechanical engineering.
Type: Journal Article
Series/ Journal Title: Analytical and bioanalytical chemistry
School: School of Mechanical and Aerospace Engineering
Rights: © 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00216-009-2833-6.
Version: Accepted version

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