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Title:
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Enhanced electrophoretic DNA separation in photonic crystal fiber.
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Author:
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Sun, Yi.; Nguyen, Nam-Trung.; Kwok, Yien Chian.
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Copyright year:
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2009 |
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Abstract:
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Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip. |
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Subject:
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DRNTU::Engineering::Mechanical engineering. |
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Type:
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Journal Article |
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Series/ Journal Title:
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Analytical and bioanalytical chemistry |
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School:
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School of Mechanical and Aerospace Engineering |
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Rights:
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© 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00216-009-2833-6. |
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Version:
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Accepted version |
