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Enhanced electrophoretic DNA separation in photonic crystal fiber

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Enhanced electrophoretic DNA separation in photonic crystal fiber

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dc.contributor.author Sun, Yi
dc.contributor.author Nguyen, Nam-Trung
dc.contributor.author Kwok, Yien Chian
dc.date.accessioned 2012-04-12T05:58:47Z
dc.date.available 2012-04-12T05:58:47Z
dc.date.copyright 2009
dc.date.issued 2012-04-12
dc.identifier.citation Sun, Y., Nguyen, N. T., & Kwok, Y. C. (2009). Enhanced electrophoretic DNA separation in photonic crystal fiber. Analytical and Bioanalytical Chemistry, 394(6), 1707-1710.
dc.identifier.uri http://hdl.handle.net/10220/7752
dc.description.abstract Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip.
dc.language.iso en
dc.relation.ispartofseries Analytical and bioanalytical chemistry
dc.rights © 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00216-009-2833-6.
dc.subject DRNTU::Engineering::Mechanical engineering.
dc.title Enhanced electrophoretic DNA separation in photonic crystal fiber
dc.type Journal Article
dc.contributor.school School of Mechanical and Aerospace Engineering
dc.identifier.doi http://dx.doi.org/10.1007/s00216-009-2833-6
dc.description.version Accepted version
dc.identifier.rims 152444

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