| dc.contributor.author |
Sun, Yi. |
| dc.contributor.author |
Nguyen, Nam-Trung. |
| dc.contributor.author |
Kwok, Yien Chian. |
| dc.date.accessioned |
2012-04-12T05:58:47Z |
| dc.date.available |
2012-04-12T05:58:47Z |
| dc.date.copyright |
2009 |
| dc.date.issued |
2012-04-12 |
| dc.identifier.citation |
Sun, Y., Nguyen, N. T., & Kwok, Y. C. (2009). Enhanced electrophoretic DNA separation in photonic crystal fiber. Analytical and Bioanalytical Chemistry, 394(6), 1707-1710. |
| dc.identifier.uri |
http://hdl.handle.net/10220/7752 |
| dc.description.abstract |
Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip. |
| dc.language.iso |
en |
| dc.relation.ispartofseries |
Analytical and bioanalytical chemistry |
| dc.rights |
© 2009 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and bioanalytical chemistry, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00216-009-2833-6. |
| dc.subject |
DRNTU::Engineering::Mechanical engineering. |
| dc.title |
Enhanced electrophoretic DNA separation in photonic crystal fiber. |
| dc.type |
Journal Article |
| dc.contributor.school |
School of Mechanical and Aerospace Engineering |
| dc.identifier.doi |
http://dx.doi.org/10.1007/s00216-009-2833-6 |
| dc.description.version |
Accepted version |
