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Nested PCR in magnetically actuated circular closed-loop PCR microchip system

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Nested PCR in magnetically actuated circular closed-loop PCR microchip system

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dc.contributor.author Lok, Khoi Seng
dc.contributor.author Lee, Peter Peng Foo
dc.contributor.author Kwok, Yien Chian
dc.contributor.author Nguyen, Nam-Trung
dc.date.accessioned 2012-05-11T08:03:32Z
dc.date.available 2012-05-11T08:03:32Z
dc.date.copyright 2012
dc.date.issued 2012-05-11
dc.identifier.citation Lok, K. S., Lee, P. P. F., Kwok, Y. C., & Nguyen, N. T. (2012). Nested PCR in magnetically actuated circular closed-loop PCR microchip system. Microchimica Acta , 177(1-2), 111-117.
dc.identifier.uri http://hdl.handle.net/10220/7905
dc.description.abstract We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of a single locus from a minute quantity of template DNA. Two sets of primers are specially designed to a target 500-bp region of the bacteriophage lambda template DNA in the first PCR run, and a 247-bp region of the targeted 500-bp first PCR product in the second PCR run. PCR is run on the microchip system and concurrently in regular thermocycler for comparison. The products are analyzed by conventional agarose gel electrophoresis. The detection limit for the initial template DNA is 1.63 × 105 copies per μL (or 8.67 pg) for the first PCR run, and 1.63 copies per μL (or 0.0867 fg) for the second run. The results are comparable to a regular thermocycler. This preliminary study opens a new gateway to future development of specialized nPCR on chip.
dc.format.extent 16 p.
dc.language.iso en
dc.relation.ispartofseries Microchimica acta
dc.rights © 2012 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Microchimica acta, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1007/s00604-012-0760-2.
dc.subject DRNTU::Engineering::Mechanical engineering.
dc.title Nested PCR in magnetically actuated circular closed-loop PCR microchip system
dc.type Journal Article
dc.contributor.school School of Mechanical and Aerospace Engineering
dc.identifier.doi http://dx.doi.org/10.1007/s00604-012-0760-2

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