Ferrofluid plug as valve and actuator for whole-cell PCR on chip

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Ferrofluid plug as valve and actuator for whole-cell PCR on chip

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dc.contributor.author Lok, Khoi Seng
dc.contributor.author Kwok, Yien Chian
dc.contributor.author Lee, Peter Peng Foo
dc.contributor.author Nguyen, Nam-Trung
dc.date.accessioned 2012-07-04T07:45:08Z
dc.date.available 2012-07-04T07:45:08Z
dc.date.copyright 2012
dc.date.issued 2012-07-04
dc.identifier.citation Lok, K. S., Kwok, Y. C., Lee, P. P. F., & Nguyen, N. T. (2012). Ferrofluid plug as valve and actuator for whole-cell PCR on chip. Sensors and Actuators B: Chemical, 166-167, (893–897).
dc.identifier.uri http://hdl.handle.net/10220/8284
dc.description.abstract Ferrofluid plug was first used as valve and actuator to circumvent the evaporation of aqueous fluid in LOC. Moreover, this dynamic plug catered for aqueous fluid expansion during its heating in the microchannel. Whole-cell polymerase chain reaction (PCR) protocol was successfully implemented in a magnetically actuated closed-loop PCR microchip system. Here, the ferrofluid plug was used to reduce the evaporation of the PCR sample in the thermal lysis step. A whole-cell PCR model was used to demonstrate this concept. The DNA plasmid, pUC19, was transformed into DH5α competent cells. Primer pairs were designed to amplify region of interest in pUC19. The transformants were cultured and selected. Whole-cell PCR was performed on intact bacteria to screen for the presence of pUC19, containing the region of interests. Serial dilutions demonstrated the limit of detection for this system to be four bacterial cells. PCR amplifications were verified with regular thermocyclers and results were comparable.
dc.format.extent 21 p.
dc.language.iso en
dc.relation.ispartofseries Sensors and actuators B: chemical
dc.rights © 2012 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Sensors and Actuators B: Chemical, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI: http://dx.doi.org/10.1016/j.snb.2012.03.001]
dc.subject DRNTU::Engineering::Mechanical engineering.
dc.title Ferrofluid plug as valve and actuator for whole-cell PCR on chip
dc.type Journal Article
dc.contributor.school School of Mechanical and Aerospace Engineering
dc.identifier.doi http://dx.doi.org/10.1016/j.snb.2012.03.001
dc.description.version Accepted version
dc.identifier.rims 164005

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