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Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes.

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Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes.

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Title: Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes.
Author: Wang, Zhijuan.; Zhang, Juan.; Chen, Peng.; Zhou, Xiaozhu.; Yang, Yanli.; Wu, Shixin.; Niu, Li.; Han, Yu.; Wang, Lianhui.; Chen, Peng.; Boey, Yin Chiang Freddy.; Zhang, Qichun.; Liedberg, Bo.; Zhang, Hua.
Copyright year: 2011
Abstract: Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10−13 M, i.e., 100 fM) and selectivity.
Subject: DRNTU::Science::Medicine::Biosensors.
Type: Journal Article
Series/ Journal Title: Biosensors and Bioelectronics
School: School of Materials Science and Engineering
Rights: © 2011 Elsevier B.V. This is the author created version of a work that has been peer reviewed and accepted for publication by Biosensors and Bioelectronics, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bios.2011.03.002].
Version: Accepted version

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