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Title:
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Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis.
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Author:
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Soo, Jian-Chow.; Zhang, Jing.; He, Qiyuan.; Agarwal, Shuchi.; Li, Hai.; Zhang, Hua.; Chen, Peng.
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Copyright year:
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2010 |
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Abstract:
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The subunit B of cholera toxin (CTB), which specifically binds with ganglioside GM1 enriched
in membrane lipid rafts, is known to interfere with multiple cell functions. However, the specific,
stable and spatially defined membrane signaling induced by CTB binding is often difficult to
investigate by applying CTB molecules in bulk solution due to quick internalization, elicited
intracellular reactions, and homogeneous interaction with the entire cell membrane. Here, we
interfaced the neuroendocrine PC12 cells with surface immobilized and patterned CTB molecules,
and interrogated the effects of CTB binding on vesicular exocytosis using integrative single-cell
study methods. It was discovered that CTB binding facilitates vesicle trafficking, docking and
exocytosis in a cholesterol dependent manner. And these effects are probably attributable to the
increased membrane GM1 and cholesterol, and enhanced Ca2+ signaling. |
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Subject:
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DRNTU::Science::Biological sciences. |
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Type:
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Journal Article |
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Series/ Journal Title:
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Integrative biology |
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School:
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School of Materials Science and Engineering |
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Rights:
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© 2010 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Integrative Biology, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1039/c0ib00006j. |
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Version:
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Accepted version |
