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Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis

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Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis

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dc.contributor.author Soo, Jianchow
dc.contributor.author Zhang, Jing
dc.contributor.author He, Qiyuan
dc.contributor.author Agarwal, Shuchi
dc.contributor.author Li, Hai
dc.contributor.author Zhang, Hua
dc.contributor.author Chen, Peng
dc.date.accessioned 2012-08-21T05:51:03Z
dc.date.available 2012-08-21T05:51:03Z
dc.date.copyright 2010
dc.date.issued 2012-08-21
dc.identifier.citation Soo, J. C., Zhang, J., He, Q., Agarwal, S., Li, H., Zhang, H., et al. (2010). Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis. Integrative Biology, 2, 250–257.
dc.identifier.uri http://hdl.handle.net/10220/8406
dc.description.abstract The subunit B of cholera toxin (CTB), which specifically binds with ganglioside GM1 enriched in membrane lipid rafts, is known to interfere with multiple cell functions. However, the specific, stable and spatially defined membrane signaling induced by CTB binding is often difficult to investigate by applying CTB molecules in bulk solution due to quick internalization, elicited intracellular reactions, and homogeneous interaction with the entire cell membrane. Here, we interfaced the neuroendocrine PC12 cells with surface immobilized and patterned CTB molecules, and interrogated the effects of CTB binding on vesicular exocytosis using integrative single-cell study methods. It was discovered that CTB binding facilitates vesicle trafficking, docking and exocytosis in a cholesterol dependent manner. And these effects are probably attributable to the increased membrane GM1 and cholesterol, and enhanced Ca2+ signaling.
dc.language.iso en
dc.relation.ispartofseries Integrative biology
dc.rights © 2010 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Integrative Biology, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1039/c0ib00006j.
dc.subject DRNTU::Science::Biological sciences
dc.title Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis
dc.type Journal Article
dc.contributor.school School of Materials Science and Engineering
dc.identifier.doi http://dx.doi.org/10.1039/c0ib00006j
dc.description.version Accepted version
dc.identifier.rims 151699

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