mirage

Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells.

DSpace/Manakin Repository

 

Search DR-NTU


Advanced Search Subject Search

Browse

My Account

Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells.

Show simple item record

dc.contributor.author Kang, Tae-Hong.
dc.contributor.author Park, Do-Young.
dc.contributor.author Choi, Yoon Ha.
dc.contributor.author Kim, Kyung-Jin.
dc.contributor.author Yoon, Ho Sup.
dc.contributor.author Kim, Kyong-Tai.
dc.date.accessioned 2012-09-13T07:04:11Z
dc.date.available 2012-09-13T07:04:11Z
dc.date.copyright 2007
dc.date.issued 2012-09-13
dc.identifier.citation Kang, T. H., Park, D. Y., Choi, Y. H., Kim, K. J., Yoon, H. S., & Kim, K. T. (2007). Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells. Molecular and Cellular Biology, 27(24), 8533-8546.
dc.identifier.issn 0270-7306
dc.identifier.uri http://hdl.handle.net/10220/8519
dc.description.abstract Mitotic chromatin condensation is essential for cell division in eukaryotes. Posttranslational modification of the N-terminal tail of histone proteins, particularly by phosphorylation by mitotic histone kinases, may facilitate this process. In mammals, aurora B is believed to be the mitotic histone H3 Ser10 kinase; however, it is not sufficient to phosphorylate H3 Ser10 with aurora B alone. We show that histone H3 is phosphorylated by vaccinia-related kinase 1 (VRK1). Direct phosphorylation of Thr3 and Ser10 in H3 by VRK1 both in vitro and in vivo was observed. Loss of VRK1 activity was associated with a marked decrease in H3 phosphorylation during mitosis. Phosphory¬lation of Ser10 by VRK1 is similar to that by aurora B. Moreover, expression and chromatin localization of VRK1 depended on the cell cycle phase. Overexpression of VRK1 resulted in a dramatic condensation of nuclei. Our findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals.
dc.language.iso en
dc.relation.ispartofseries Molecular and cellular biology
dc.rights © 2007 American Society for Microbiology. This is the author created version of a work that has been peer reviewed and accepted for publication by Molecular and Cellular Biology, American Society for Microbiology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1128/MCB.00018-07].
dc.subject DRNTU::Science::Biological sciences::Molecular biology.
dc.title Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells.
dc.type Journal Article
dc.contributor.school School of Biological Sciences
dc.identifier.doi http://dx.doi.org/10.1128/MCB.00018-07
dc.description.version Accepted version

Files in this item

Files Size Format View
18. Mitotic His ... e 1 in Mammalian Cells.pdf 869.4Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Statistics

Total views

All Items Views
Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cells. 195

Total downloads

All Bitstreams Views
18. Mitotic Histone H3 Phosphorylation by Vaccinia-Related Kinase 1 in Mammalian Cells.pdf 93

Top country downloads

Country Code Views
China 33
United States of America 28
Singapore 20
Russian Federation 3
Japan 2

Top city downloads

city Views
Beijing 27
Mountain View 25
Singapore 20
Boca Raton 1
Houston 1

Downloads / month

  2014-02 2014-03 2014-04 total
18. Mitotic Histone H3 Phosphorylation by Vaccinia-Related Kinase 1 in Mammalian Cells.pdf 0 0 4 4