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Immobilization of recombinant vault nanoparticles on solid substrates

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Immobilization of recombinant vault nanoparticles on solid substrates

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Title: Immobilization of recombinant vault nanoparticles on solid substrates
Author: Xia, Yun; Ramgopal, Yamini; Li, Hai; Shang, Lei; Srinivas, Parisa; Kickhoefer, Valerie A.; Rome, Leonard H.; Preiser, Peter Rainer; Boey, Freddy Yin Chiang; Zhang, Hua; Venkatraman, Subbu S.
Copyright year: 2010
Abstract: Native vaults are nanoscale particles found abundantly in the cytoplasm of most eukaryotic cells. They have a capsule-like structure with a thin shell surrounding a “hollow” interior compartment. Recombinant vault particles were found to self-assemble following expression of the major vault protein (MVP) in a baculovirus expression system, and these particles are virtually identical to native vaults. Such particles have been recently studied as potential delivery vehicles. In this study, we focus on immobilization of vault particles on a solid substrate, such as glass, as a first step to study their interactions with cells. To this end, we first engineered the recombinant vaults by fusing two different tags to the C-terminus of MVP, a 3 amino acid RGD peptide and a 12 amino acid RGD-strep-tag peptide. We have demonstrated two strategies for immobilizing vaults on solid substrates. The barrel-and-cap structure of vault particles was observed for the first time, by atomic force microscopy (AFM), in a dry condition. This work proved the feasibility of immobilizing vault nanoparticles on a material surface, and the possibility of using vault nanoparticles as localized and sustainable drug carriers as well as a biocompatible surface moiety.
Subject: DRNTU::Engineering::Materials.
Type: Journal Article
Series/ Journal Title: ACS nano
School: School of Materials Science and Engineering
Rights: © 2010 American Chemical Society.

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