Immobilization of recombinant vault nanoparticles on solid substrates

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Immobilization of recombinant vault nanoparticles on solid substrates

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dc.contributor.author Xia, Yun
dc.contributor.author Ramgopal, Yamini
dc.contributor.author Li, Hai
dc.contributor.author Shang, Lei
dc.contributor.author Srinivas, Parisa
dc.contributor.author Kickhoefer, Valerie A.
dc.contributor.author Rome, Leonard H.
dc.contributor.author Preiser, Peter Rainer
dc.contributor.author Boey, Freddy Yin Chiang
dc.contributor.author Zhang, Hua
dc.contributor.author Venkatraman, Subbu S.
dc.date.accessioned 2012-09-24T01:34:30Z
dc.date.available 2012-09-24T01:34:30Z
dc.date.copyright 2010
dc.date.issued 2012-09-24
dc.identifier.citation Xia, Y., Ramgopal, Y., Li, H., Shang, L., Srinivas, P., Kickhoefer, V. A., et al. (2010). Immobilization of recombinant vault nanoparticles on solid substrates. ACS Nano, 4(3), 1417-1424.
dc.identifier.issn 1936-0851
dc.identifier.uri http://hdl.handle.net/10220/8608
dc.description.abstract Native vaults are nanoscale particles found abundantly in the cytoplasm of most eukaryotic cells. They have a capsule-like structure with a thin shell surrounding a “hollow” interior compartment. Recombinant vault particles were found to self-assemble following expression of the major vault protein (MVP) in a baculovirus expression system, and these particles are virtually identical to native vaults. Such particles have been recently studied as potential delivery vehicles. In this study, we focus on immobilization of vault particles on a solid substrate, such as glass, as a first step to study their interactions with cells. To this end, we first engineered the recombinant vaults by fusing two different tags to the C-terminus of MVP, a 3 amino acid RGD peptide and a 12 amino acid RGD-strep-tag peptide. We have demonstrated two strategies for immobilizing vaults on solid substrates. The barrel-and-cap structure of vault particles was observed for the first time, by atomic force microscopy (AFM), in a dry condition. This work proved the feasibility of immobilizing vault nanoparticles on a material surface, and the possibility of using vault nanoparticles as localized and sustainable drug carriers as well as a biocompatible surface moiety.
dc.language.iso en
dc.relation.ispartofseries ACS nano
dc.rights © 2010 American Chemical Society.
dc.subject DRNTU::Engineering::Materials
dc.title Immobilization of recombinant vault nanoparticles on solid substrates
dc.type Journal Article
dc.contributor.school School of Materials Science and Engineering
dc.identifier.doi http://dx.doi.org/10.1021/nn901167s

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