The specificity and catalytic properties of Alu I methylase

DSpace/Manakin Repository


Search DR-NTU

Advanced Search Subject Search


My Account

The specificity and catalytic properties of Alu I methylase

Show full item record

Title: The specificity and catalytic properties of Alu I methylase
Author: Yoon, Ho Sup; Suh, Hyang; Kim, Kitae; Han, Moon H.; Yoo, Ook Joon
Copyright year: 1985
Abstract: The specific methylation site for Alu I methylase was the cytosine nucleotide in Alu I sequence. The position of the methylated cytosine nucleotide was determined by the chemical cleavage reactions of the Maxam-Gilbert DNA sequencing procedure. As expected, the methylated cytosine nucleotide bands were disappeared on C+ T and C lanes on 12% sequencing gels. Alu I methylase was maximally active at near pH 7.5 in the presence of 50 mM NaCl. The methylase did not require Mg++ for activity, and obeyed Michaelis-Menten Kinetics with respect to both AdoMet and DNA. At 37°C, the Km for AdoMet was 0.44 μM, that for the Alu I site of pBR 322 DNA was 4.03 nM, and the corresponding turnover numbers were 1.83 methyl transfer per minute per monomer and 1.61 transfers per minute per monomer, respectively.
Subject: DRNTU::Science::Biological sciences.
Type: Journal Article
Series/ Journal Title: Korean biochemistry journal
School: School of Biological Sciences
Rights: © 1985 Springer Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Korean Biochem. J., Springer Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://www.jbmb.or.kr/jbmbonline/18_1/list.html].
Version: Accepted version

Files in this item

Files Size Format View
54.The Specific ... ies of Alu I Methylase.pdf 300.3Kb PDF View/Open

SFX Query

- Get published version (via NTU subscribed resources)

This item appears in the following Collection(s)

Show full item record


Total views

All Items Views
The specificity and catalytic properties of Alu I methylase 462

Total downloads

All Bitstreams Views
54.The Specificity and Catalytic Properties of Alu I Methylase.pdf 258

Top country downloads

Country Code Views
China 98
United States of America 98
Ukraine 19
Singapore 9
Russian Federation 7

Top city downloads

city Views
Mountain View 61
Beijing 48
Singapore 9
Sunnyvale 7
Redwood City 6