Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/95346
Title: | Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF) | Authors: | Lin, Jie Chen, Hu Dröge, Peter Yan, Jie |
Keywords: | DRNTU::Science::Biological sciences::Genetics | Issue Date: | 2012 | Source: | Lin, J., Chen, H., Dröge, P., & Yan, J. (2012). Physical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF). PLoS ONE, 7(11), e49885. | Series/Report no.: | PLoS ONE | Abstract: | The integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting that non-specific binding of IHF to DNA plays a role in the physical organization of bacterial chromatin. However, it is unclear how non-specific DNA association contributes to DNA organization. By using a combination of single DNA manipulation and atomic force microscopy imaging methods, we show here that distinct modes of non-specific DNA binding of IHF result in complex global DNA conformations. Changes in KCl and IHF concentrations, as well as tension applied to DNA, dramatically influence the degree of DNA-bending. In addition, IHF can crosslink DNA into a highly compact DNA meshwork that is observed in the presence of magnesium at low concentration of monovalent ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation. | URI: | https://hdl.handle.net/10356/95346 http://hdl.handle.net/10220/9317 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0049885 | Schools: | School of Biological Sciences | Rights: | © 2012 The Authors. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
3. Physical Organization of DNA.pdf | 832.22 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
10
36
Updated on Mar 18, 2024
Web of ScienceTM
Citations
10
32
Updated on Oct 27, 2023
Page view(s) 5
917
Updated on Mar 18, 2024
Download(s) 10
387
Updated on Mar 18, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.