Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/52905
Title: | Characterization of dendritic cells expressing the glycoprotein Plet-1. | Authors: | Teo, Zi Yang. | Keywords: | DRNTU::Science::Biological sciences::Molecular biology | Issue Date: | 2013 | Abstract: | Dendritic cells (DCs) are vital Antigen-Presenting Cells (APCs) specialized in capturing, processing and presenting of self or foreign antigens to immune cells such as naïve T cells. They are important mediators between the innate and adaptive immune. Also, they help maintain the immune balance between tolerogenicity and immunogenicity. Placenta expressed transcript-1 (Plet-1) is an orphan glycoprotein found to be expressed on various cells and organs. However, the function of these Plet-1 associated cells and organs still remain unclear. In Ruedl’s Laboratory, for the very first time, Plet-1 mRNA was found to be the most highly expressed in Granulocyte-Macrophage Colony-Stimulated Factor (GMCSF) stimulated DCs. In addition, the existence of DCs expressing Plet-1 on lymphoid organs such as the peripheral lymph nodes, mesenteric lymph nodes, peyer’s patches and colonic lamina propria (cLP) was also later proven. To further characterize Plet-1+ DCs, in this study for the first time, we investigated on the Plet-1+ DCs using the Dextran Sodium Sulphate (DSS)-induced colitis model to induce intestinal inflammation on mice. We aim to elucidate whether Plet-1+ DCs are recruited or enhanced during inflammation. Our results revealed that Plet-1+ DCs show a strong indication towards playing a role in inflammation and that GMCSF stimulation is important in facilitating the production and upregulation of Plet-1+ DCs. In addition, we proved that the age of the mice may not be a contributing factor towards the fluctuating Plet-1+ DC population among repeats. We speculate that Plet-1+ DC play a critical role intestinal inflammation such as in colitis. | URI: | http://hdl.handle.net/10356/52905 | Schools: | School of Biological Sciences | Rights: | Nanyang Technological University | Fulltext Permission: | restricted | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Student Reports (FYP/IA/PA/PI) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
SBS12-220.pdf Restricted Access | FYP Thesis (Main article) | 2.54 MB | Adobe PDF | View/Open |
Page view(s) 10
760
Updated on Mar 28, 2024
Download(s)
7
Updated on Mar 28, 2024
Google ScholarTM
Check
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.