Academic Profile

Professional Experience

Junior Group Leader, DFG Research Center for Experimental Biomedicine, University of Wuerzburg, Germany
Postdoctoral Studies, Gene Center, LMU Munich, Germany
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Asst Prof Shashi Bhushan
Assistant Professor, School of Biological Sciences

We are interested in determining 3D structures of macromolecular protein complexes to understand their function and mechanism. We employ Cryo-Electron Microscopy with single particle reconstruction as our main tool for structural determination. Our laboratory is equipped with advanced Electron Microscopes and high speed computing clusters for routine single particle reconstruction work. We are mainly interested in following research areas:


Protein translocation across cellular membranes
Co-translational protein folding
Protein secretion
Translational stalling and regulation
 
  • Biochemical And Structural Investigations Of The Mycobacterial MmpL3 Transporter

  • Cooperative modulation of E. coli aquaporin water channel by cardiolipin: a new paradigm for allosteric control of multi-subunit membrane proteins.

  • Functional and structural characterization of an exemplar potassium ion channel from Mycobacterium tuberculosis

  • Mechanism and Compartmentalization of CbbQO-Type Rubisco Activases

  • Reconstituting phase separations that enable the microalgal CO2 superchargers
 
  • Wong CF, Shin J, Manimekalai MSS, Saw WG, Zhan Y, Bhushan S, Kumar A, Ragunathan P, Grüber G. (2017). The uniqueness of AhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C. Scientific Reports, 7, 5159.

  • Kumar V, Ero R, Ahmed T, Goh KJ, Zhan Y, Bhushan S, and Gao YG. (2016). The Structure of Elongation Factor 4 (EF4/LepA) in GTP Form Bound to the Ribosome. Journal of Biological Chemistry, 291(Cover Image July), 12943-50.

  • Singh D., Sielaff H., Sundararaman L., Bhushan S., Grüber G. (2015). The stimulating role of subunit F in ATPase activity inside the A1-complex of the Methanosarcina mazei Gö1 A1AO ATP synthase. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1857, 177-187.

  • Saha A, IChakraborty I, Kraft C, Bhushan S and Ghosh S. (2013). Microtubule nucleation from a functionalised SiO2 EM grid. RSC Advances, 3, 7688–7691.

  • Winzer A, Kraft C, Bhushan S, Stepanenko V and Tessmer I. (2012). Correcting for AFM tip induced topography convolutions in protein-DNA samples. Ultramicroscopy, 121, 08-15.