Academic Profile

Zbynek Bozdech holds currently a position as Assistant Professor at the School of Biological Sciences at Nanyang Technological University in Singapore leading a research team investigating molecular aspects of malaria parasite life cycle. In 1990, he has received his masters’ degree in Biochemistry from Charles University in Prague. From here he moved to McGill University in Montréal Canada to start his PhD studies involving protein trafficking in Plasmodium infected erythrocytes. In 1998 he moved to UCSF, where he worked in the laboratory of Joseph L. DeRisi exploring the transcriptome of Plasmodium falciparum using the fast emerging microarray technology. This work has lead to assembly of one of the first P. falciparum microarray and explorations of the global transcription pattern of the P. falciparum intraerythrocytic developmental cycle. In 2004, he moved to the newly forming School of Biological Sciences at NTU, to start his own research group. Presently Dr Bozdech is exploring several molecular aspects of translation and posttranslational regulation associated with the progression of the P. falciparum life cycle. In addition, using several technologies of high throughout genomics and proteomics his group is exploring molecular mechanisms associated with virulence and drug resistance in field strains of P. falciparum and P. vivax.
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Prof Zbynek Bozdech
Professor, School of Biological Sciences

My research focuses on regulatory mechanisms that are associated with the progression of the complex life cycle of human malaria parasites, Plasmodium species. The research activities can be divided into three major objectives

(i) Characterizations of transcriptional regulation in Plasmodium. In these studies we conduct genome-wide transcriptional profiling of field isolates and chemical genomics analyses of the laboratory strains. The main goal of these studies is to identify transcriptional regulation that controls parasites virulence as well as responses to drug therapies.

(ii) Quantitative proteomics of the Plasmodium intraerythrocytic developmental cycle. The main objective these studies is to characterize protein abundance profiles as well as the pattern of posttranslational modification associated with the progression of the Plasmodium erythrocytic development. These studies are conducted in the proteome-wide manner using the two dimensional gel electrophoresis approaches. The main objective is to identify regulatory elements of Plasmodium translational machinery as new therapeutic targets for human malaria.

(iii) Epigenetic regulation of transcriptional control in Plasmodium parasites. Using a chemical genetic approach we characterize a role of Histone Deacetylases (HDAC) in the transcriptional regulation of the Plasmodium life cycle. Using these approaches we attempt to analyze the histone code in Plasmodium and its role in parasite growth and development. Simultaneously we evaluate a potential of HDAC inhibitors as new malaria chemotherapeutics.
 
  • Discovering New Target Space for the Development of Drugs Against Malaria Parasites

  • Discovering New Target Space for the Development of Drugs Against Malaria Parasites (Zbynek Bozdech)

  • Mechanisms of Zoonosis of Infectious Diseases: The Malaria Model

  • Mechanisms of Zoonosis of Infectious Diseases: The Malaria Model (NUS PI: Benoit Malleret, Kevin Tan Shyong Wei and Juan Pablo Bifani)

  • Protein Drug target Identifications in Human Malaria Parasites
 
  • Dziekan, J.M., Wirjanata, G., Dai, L., Go, K.D., Yu, H., Lim, Y.T., Chen, L., Wang, L.C., Puspita, B., Prabhu, N., Sobota R. M., Nordlund P., Bozdech Z. (2020). Cellular thermal shift assay for the identification of drug–target interactions in the Plasmodium falciparum proteome. Nature Protocols, , 1-41.

  • Lei Zhu, Jaishree Tripathi, Frances Maureen Rocamora, Olivo Miotto, Rob van der Pluijm, Till S. Voss, Sachel Mok, Dominic P. Kwiatkowski, François Nosten, Nicholas P. J. Day, Nicholas J. White, Arjen M. Dondorp, Zbynek Bozdech & Tracking Resistance to Artemisinin Collaboration I. (2018). The origins of malaria artemisinin resistance defined by a genetic and transcriptomic background. Nature Communications, 9(1), 5158.

  • Gupta AP, Zhu L, Tripathi J, Kucharski M, Patra A, Bozdech Z. (2017). Histone 4 lysine 8 acetylation regulates proliferation and host-pathogen interaction in Plasmodium falciparum. Epigenetics & Chromatin, 22(10), 40.

  • Zhu L, Mok S, Imwong M, Jaidee A, Russell B, Nosten F, Day NP, White NJ, Preiser PR, Bozdech Z. (2016). New insights into the Plasmodium vivax transcriptome using RNA-Seq. Scientific Reports. Scientific Reports, .

  • Con Dogovski, Stanley C. Xie, Gaetan Burgio, Jess Bridgford, Sachel Mok, James M. McCaw, Kesinee Chotivanich, Shannon Kenny, Nina Gnädig, Judith Straimer, Zbynek Bozdech, David A. Fidock, Julie A. Simpson, Arjen M. Dondorp, Simon Foote, Nectarios Klonis, Leann Tilley. (2015). Targeting the Cell Stress Response of Plasmodium falciparum to Overcome Artemisinin Resistance. PLos Biology, 13(4).