Academic Profile

Date of 1st Appointment: 11th of August 2005
Present Appointment: Associate Professor

● Academic Qualification
2002 Ph.D. (habil.) in Biochemistry and Molecular Biology, University of the Saarland, Germany
2001 Ph.D. (habil.) in Biochemistry, University of Osnabrück, Germany
1995 Ph.D. (rer. nat.) in Biochemistry, Johannes Gutenberg-University Mainz, Germany
1992 Diploma Degree, Johannes Gutenberg-University Mainz, Germany

● Awards
1995 - 1996, Postdoc-Fellowship of the German academical exchange service (DAAD)
1997 - 1999, Fellowship of the Human Frontiers Science Programs (HFSP)
2000 - 2001, Habilitation-Fellow of the German Research Foundation (DFG)

● Administrative involvements
1. Deputy Head of Division of Structural and Computational Biology, School of Biological Sciences,
Nanyang Technological University
2. Member of the Steering committee of the Singapore Bioimaging Consortium (SBIC)
ggrueber_1_2.JPG picture
Prof Gerhard Gruber
Professor, School of Biological Sciences

Research Activities
The research group on Structure and function of molecular motors of A/Prof. G. Gruber in the Division of Structural and Computational Biology, SBS, NTU, is recognized for their expertise in determining the relationships between the structure and function of the so-called A1AO ATP synthases, V1VO ATPases, F1FO ATP synthases and AAA-ATPases. These enzymes are proposed to be the smallest biological motor proteins (nano-motor proteins). In order to get inside into the structure of these multi-subunit membrane complexes techniques like single particle analysis of electron micrographs, solution X-ray scattering, X-ray crystallography and NMR-spectroscopy are used. The functional and dynamical processes inside these enzymes are studied by fluorescence spectroscopy (e.g. FRET, FCS, intrinsic fluorescence spectroscopy) and biochemical approaches.
  • Mycobacterium abscessus L-aspartate a-decarboxylase, an attractive target for compound development


  • Target Based Discovery Of Next Generation Pyrazinamide

  • Targeting Oxidative Phosphorylation For The Rational Development of Sterilising Drug Combination For Drug-Resistant Tuberculosis
  • Saw, W.-G., Wong, C.-F., Dick, T., Grüber, G. (2020). Overexpression, purification, enzymatic and microscopic characterization of recombinant mycobacterial F-ATP synthase. Biochemical and Biophysical Research Communications, 522(2), 374-380.

  • Saw, W.-G., Wu, M.-L, Ragunathan, P., Biuković, G, Lau, A.-M., Shin, J., Harikishore, A., Cheung, C.-Y., Hards, K., Sarathy, J. P., Bates, R. W., Cook, G. M. C., Dick, T., and Grüber, G. (2019). Disrupting coupling within mycobacterial F-ATPsynthase subunit ε causes dysregulated energy production and cell wall biosynthesis. Scientific Reports, 9, 16759.

  • Kamariah, N., Ragunathan, P., Shin, J., Saw, W.-G., Wong, C.F., Dick, T., and Grüber, G. (2019). Unique structural and mechanistic properties of mycobacterial F-ATP synthases: Implications for drug design. Progress in Biophysics & Molecular Biology, in press.

  • Saw, W.G., Pan, A., Manimekalai, M.S.S., Grüber, A., and Grüber, G. (2019). Structure and flexibility of Non-structural proteins 3 and -5 of Dengue- and Zika viruses in solution. Progress in Biophysics & Molecular Biology, 143, 67-77.

  • Saw, W.G., Chan, K., Vasudevan, S.G. and Grüber, G. (2019). Zika virus non-structural protein 5 residue 681 is critical for dimer formation and enzymatic activity. FEBS Letters, 593(12), 1272-1291.