Academic Profile : Faculty

zxliang_1_2.JPG picture
Prof Liang Zhao-Xun
Professor, School of Biological Sciences
External Links
 
Education
Ph.D., Chemistry, Northwestern University, USA (with Brain Hoffman)
B.S./M.S., Organic Chemistry, Lanzhou University, China

Employment
Sep 2005 - Present, Assistant, associate and full professor, School of Biological Sciences, Nanyang Technological University, Singapore
Jan 2002 - Jul 2005, Postdoc fellow, Department of Chemistry, University of California at Berkeley (with Judith Klinman)
My research interests lie broadly at the interface of chemistry and biology. The main thrust of my group is to discover novel enzymes and antimicrobial targets. The two main research projects in our lab are:

Project 1. Soil and marine microorganisms produce a wide variety of secondary metabolites with spectacular chemical structure and potent biological activity. Relying on a genome-guided approach, we focus on discovering novel biosynthetic enzymes and secondary metabolites encoded by unusual biosynthetic gene clusters. After isolating phylogenetically unique soil and marine microbes from the tropic environment of South East Asia, we usually sequence and mine the genome of the microbes to identify novel biosynthetic pathways. We employ a variety of genetic, biochemical, and synthetic biology tools to manipulate the biosynthetic gene clusters to produce (and modify) the secondary metabolites and characterize the function and mechanism of the novel biosynthetic enzymes. We expect the research work to yield novel bioactive natural products with potential therapeutic values and enzymes that can be exploited as biocatalysts.

Project 2. Cyclic dinucleotides (e.g., c-di-GMP and c-di-AMP) have emerged as prominent messengers in many multidrug-resistant pathogenic bacteria in recent years. Cyclic dinucleotides contribute to bacterial pathogenesis by mediating such important processes as virulence expression, antibiotic resistance, and biofilm formation. Accumulating evidence suggests that the cellular level of cyclic dinucleotides is controlled in a spatiotemporal manner by a complex network of enzymes and effector proteins. We are conducting biochemical, structural, and transcriptomic studies to understand cyclic dinucleotide-mediated mechanisms that underpin bacterial infection and antibiotic resistance. Given the vital role played by the cyclic nucleotides in both acute and chronic bacterial infection, we expect the research to disclose novel signaling proteins and pathways that can be targeted for developing antimicrobial and biofilm-controlling agents.

A full list of my publications can be found at https://scholar.google.com/citations?hl=en&user=F-DuOAkAAAAJ&view_op=list_works.Please contact me directly (zxliang@ntu.edu.sg) if you are interested in joining us.
 
  • Engineering Solutions Inspired by Plastics-eating Worms to End Plastic Waste
  • Heterologous Production Of Cannabinoids In Proprietary Streptomyces Hosts
  • Specialized metabolites from the human gut microbiota
US 2012/0040403 A1: A Diguanylate Cyclase, Method of Producing the Same and its Use in the Manufacture of Cyclic-DI-GMP and Analogues Thereof (2014)
Abstract: Described is a new stand-alone diguanylate cyclase polypeptide having a GGDEF motif and a mutated I-site that does not bind c-di-GMP. We demonstrate that the production yield of c-di-GMP and analogues was significantly increased by mutation of a conserved residue in the putative regulatory I-site.