Academic Profile

Guillaume Thibault is an Assistant Professor and Principal Investigator of the Lipid Regulation and Cell Stress Laboratory at the School of Biological Sciences, Nanyang Technological University, Singapore. He is also an Adjunct Principal Investigator at the Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR). He obtained his PhD degree from the University of Toronto, Canada in 2007 and subsequently he carried out a postdoctoral fellowship in the laboratory of Dr. Davis Ng at the Temasek Life Sciences Laboratory, National University of Singapore. During that time, Guillaume research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) Postgraduate Scholarship and the Singapore Millennium Foundation Post-Doctoral Fellowship. In 2013, Guillaume received The Elite Nanyang Assistant Professorship Award when joining Nanyang Technological University to establish his independent research group. Guillaume is an expert on cellular stress responses in the context of lipid bilayer stress. His research group mostly focus on elucidating the role of the endoplasmic reticulum in sensing and maintaining lipid and protein homeostasis in the context of metabolic diseases and aging.

ACADEMIC QUALIFICATIONS
2013 Post-doctoral Research Fellow, Temasek Life Sciences Laboratory, National University of Singapore
2007 Ph.D. in Biochemistry, University of Toronto, Canada
2002 B.Sc. in Biochemistry, Université du Québec à Montréal, Canada
1999 Diploma in Analytical Chemistry, Collège Ahuntsic, Canada
thibault_1_2.JPG picture
Asst Prof Guillaume Thibault
Assistant Professor, School of Biological Sciences

LIPID REGULATION IN EUKARYOTES

Hundreds of distinct lipids, of varying concentrations, assemble to form biological membranes. The most abundant, phospholipids, varies according to head group structures, acyl chain length and double bounds. In eukaryotes, lipid compositions can differ widely among organelles. In most cases, the biological significance of these differences remains unclear

The complex organization of cellular membranes suggests the need of sophisticated homeostatic regulatory mechanisms. Links were made with an endoplasmic reticulum (ER) stress pathway called the unfolded protein response (UPR). The UPR activation is required to ease the damaging effects of ER stress. Yeast relies exclusively on the Ire1p pathway while metazoans have two additional UPR outputs. This characteristic makes budding yeast a very attractive model organism since its sole UPR pathway can be easily manipulated. Normally, this response leads to ER homeostasis by facilitating refolding of proteins and enhancing recognition and degradation of misfolded proteins. Meant to be temporary, the UPR must be deactivated to avoid cell death due to chronic ER stress. Many diseases, such as Alzheimer, Parkinson, diabetes mellitus type 2, and hepatic steatosis, have been linked to recurrent ER stress.
 
  • Characterising The Anti-Aging Role Of The UPR From High Glucose Diet

  • Dissecting the neuroprotective role of WDFY3 to counteract age-mediated decline in proteostasis.

  • Endomembrane System Architecture, Dynamics, and Functions in Aging Cells and Tissues (PI Guillaume Thibault)

  • Identify Essential Genes To Construct A Functional Unfolded Protein Response Programme Using Synthetic Biology

  • Targeting Host-Pathogen Synergy in Wound Infection
 
  • K. Rizzolo, A.Y.H. Yu, A. Ologbenla, S.R. Kim, H. Zhu, K. Ishimori, Guillaume Thibault , E. Leung, Y.W. Zhang, M. Teng, M. Haniszewski, N. Miah, S. Phanse, Z. Minic, S. Lee, J.D. Caballero, M. Babu, F.T.F. Tsai, T. Saio, W.A. Houry. Functional cooperativity between the trigger factor chaperone and the ClpXP proteolytic complex. Nat Commun 2021 Jan 12. doi. 10.1038/s41467-020-20553-x

  • Wei Sheng Yap, Peter Shyu Jr., Maria Laura Gaspar, Stephen A. Jesch, Charlie Marvalim, William A. Prinz, Susan A. Henry, Guillaume Thibault. The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis. J Cell Sci 2020 Nov 5. doi. ​10.1242/jcs.248526

  • Nurulain Ho, Wei Sheng Yap, Jiaming Xu, Haoxi Wu, Jhee Hong Koh, Wilson W.B. Goh, Bhawana George, Shu Chen Chong, Stefan Taubert, Guillaume Thibault. ​Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress. J Cell Biol 2020 Apr 29. doi. 10.1083/jcb.201909165

  • Rachel S. Kraut, Guillaume Thibault, Sarita Hebbar, Elisabeth Knust, eds. Connections to Membrane Trafficking Where You Least Expect Them: Diseases, Dynamics, Diet and Distance. Frontiers Media SA. doi. 10.3389/978-2-88963-429-3

  • Xiu Hui Fun and Guillaume Thibault. Lipid bilayer stress and proteotoxic stress-induced unfolded protein response deploy divergent transcriptional and non-transcriptional programmes. Biochim Biophys Acta, Mol Cell Biol Lipids 2020 Jan doi. 10.1016/j.bbalip.2019.04.009

  • Sarita Hebbar, Elisabeth Knust, Guillaume Thibault, Rachel Kraut. Editorial: Connections to Membrane Trafficking Where You Least Expect Them: Diseases, Dynamics, Diet and Distance. Front Cell Dev Biol 2019 Dec 6. doi. 10.3389/fcell.2019.00327

  • Wilson W.B. Goh, Subhash Thalappilly, Guillaume Thibault. Moving beyond the current limits of data analysis in longevity and healthy lifespan studies. Drug Discov Today 2019 Dec. doi. 10.1016/j.drudis.2019.08.008

  • Kizito Njah, Sayan Chakraborty, Beiying Qiu, Surender Arumugam, Anandhkumar Raju, Ajaybabu V. Pobbati, Manikandan Lakshmanan, Vinay Tergaonkar, Guillaume Thibault, Xiaomeng Wang, Wanjin Hong. A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis. Cell Rep 2019 Jul 23. doi. 10.1016/j.celrep.2019.06.036

  • Peter Shyu Jr, Benjamin S.H. Ng, Nurulain Ho, Ruijie Chaw, Yi Ling Seah, Charlie Marvalim, Guillaume Thibault. Membrane phospholipid alteration causes chronic ER stress through early degradation of homeostatic ER-resident proteins. Sci Rep 2019 Jun 14. doi. 10.1038/s41598-019-45020-6

  • Caroline Beaudoin-Chabot, Lei Wang, Alexey V. Smarun, Dragoslav Vidovic, Mikhail S. Shchepinov, Guillaume Thibault. Deuterated polyunsaturated fatty acids reduce oxidative stress and extend the lifespan of C. elegans​. Front Physiol 2019 May 28. doi. 10.3389/fphys.2019.00641

  • Jhee Hong Koh, Lei Wang, Caroline Beaudoin-Chabot, Guillaume Thibault. ​Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress. J Cell Sci 2018 Nov 21. doi. 10.1242/jcs.217992

  • Peter Shyu Jr, Xing Fah Wong, Karen Crasta, Guillaume Thibault. ​Dropping in on lipid droplets: insights into cellular stress and cancer. Biosci Rep 2018 Sep 19, 38(5). doi.10.1042/BSR20180764

  • Nurulain Ho, Chengchao Xu, Guillaume Thibault. From the unfolded protein response to metabolic diseases - lipids under the spotlight. J Cell Sci 2018 Feb 8;131(3). doi. 10.1242/jcs.199307

  • Haoxi Wu, Benjamin S.H. Ng, Guillaume Thibault. Endoplasmic Reticulum Stress Response in Yeast and Humans. Biosci Rep 2014 Aug 1, 34(4). doi. 10.1042/BSR20140058

  • Chengchao Xu, Songyu Wang, Guillaume Thibault, Davis T. Ng. Futile Protein Folding Cycles in the ER Are Terminated by the Unfolded Protein O-Mannosylation Pathway. Science 340, 978-981. doi. 10.1126/science.1234055

  • Guillaume Thibault and Davis T. Ng. The Endoplasmic Reticulum-Associated Degradation Pathways of Budding Yeast. Cold Spring Harb Perspect Biol 2012 Dec 3;4(12):a013193. doi. 10.1101/cshperspect.a013193

  • Guillaume Thibault , Guanghou Shui, Woong Kim, Graeme C. McAlister, Nurzian Ismail, Steven P. Gygi, Markus R. Wenk, Davis T. Ng. The membrane stress response buffers lethal effects of lipid disequilibrium by reprogramming the protein homeostasis network. Mol Cell 48, 16-27. doi. 10.1016/j.molcel.2012.08.016