Nanoporous carbon as a sensing platform for DNA detection : the use of impedance spectroscopy for hairpin-DNA based assay
Poh, Hwee Ling
Date of Issue2012
School of Physical and Mathematical Sciences
Nanoporous carbons represent well defined materials with large surface area, defined porosity, high electrical conductivity and fast heterogeneous electron transfer rates. These materials are seldom used for sensing and even more rarely for biosensing. We for the first time show the application of nanoporous carbon using electrochemical impedance spectroscopy technique to detect DNA hybridization and polymorphism. Our findings demonstrated the proof of concept of using nanoporous carbon in nucleic acid analysis, being able to detect the presence of a single nucleotide polymorphism.
© 2012 The Royal Society of Chemistry. This paper was published in RSC Advances and is made available as an electronic reprint (preprint) with permission of The Royal Society of Chemistry. The paper can be found at the following official DOI: [http://dx.doi.org/10.1039/C1RA00812A]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.