dc.contributor.authorLoo, Adeline Huiling
dc.contributor.authorBonanni, Alessandra
dc.contributor.authorAmbrosi, Adriano
dc.contributor.authorPoh, Hwee Ling
dc.contributor.authorPumera, Martin
dc.identifier.citationLoo, A. H., Bonanni, A., Ambrosi, A., Poh, H. L., & Pumera, M. (2012). Impedimetric immunoglobulin G immunosensor based on chemically modified graphenes. Nanoscale, 4(3), 921-925.en_US
dc.description.abstractImmunosensors which display high sensitivity and selectivity are of utmost importance to the biomedical field. Graphene is a material which has immense potential for the fabrication of immunosensors. For the first time, we evaluate the immunosensing capabilities of various graphene surfaces in this work. We propose a simple and label-free electrochemical impedimetric immunosensor for immunoglobulin G (IgG) based on chemically modified graphene (CMG) surfaces such as graphite oxide, graphene oxide, thermally reduced graphene oxide and electrochemically reduced graphene oxide. Disposable electrochemical printed electrodes were first modified with CMG materials before anti-immunoglobulin G (anti-IgG), which is specific to IgG, was immobilized. The principle of detection lies in the changes in impedance spectra of the redox probe after the attachment of IgG to the immobilized anti-IgG. It was found that thermally reduced graphene oxide has the best performance when compared to the other CMG materials. In addition, the optimal concentration of anti-IgG to be deposited onto the modified electrode surface is 10 μg ml−1 and the linear range of detection of the immunosensor is from 0.3 μg ml−1 to 7 μg ml−1. Finally, the fabricated immunosensor also displays selectivity for IgG.en_US
dc.rights© 2012 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Nanoscale, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C2NR11492E].en_US
dc.subjectDRNTU::Engineering::Materials::Nanostructured materials
dc.titleImpedimetric immunoglobulin G immunosensor based on chemically modified graphenesen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.description.versionAccepted version

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