Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/96332
Title: The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export
Authors: Lu, L.
Hannoush, R. N.
Goess, B. C.
Varadarajan, S.
Shair, M. D.
Kirchhausen, Tomas.
Issue Date: 2013
Source: Lu, L., Hannoush, R. N., Goess, B. C., Varadarajan, S., Shair, M. D., & Kirchhausen, T. (2013). The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export. Molecular Biology of the Cell, 24(7), 1020-1029.
Series/Report no.: Molecular biology of the cell
Abstract: The mammalian endoplasmic reticulum (ER) is an organelle that maintains a complex, compartmentalized organization of interconnected cisternae and tubules while supporting a continuous flow of newly synthesized proteins and lipids to the Golgi apparatus. Using a phenotypic screen, we identify a small molecule, dispergo, that induces reversible loss of the ER cisternae and extensive ER tubulation, including formation of ER patches comprising densely packed tubules. Dispergo also prevents export from the ER to the Golgi apparatus, and this traffic block results in breakdown of the Golgi apparatus, primarily due to maintenance of the constitutive retrograde transport of its components to the ER. The effects of dispergo are reversible, since its removal allows recovery of the ER cisternae at the expense of the densely packed tubular ER patches. This recovery occurs together with reactivation of ER-to-Golgi traffic and regeneration of a functional Golgi with correct morphology. Because dispergo is the first small molecule that reversibly tubulates the ER and inhibits its export function, it will be useful in studying these complex processes.
URI: https://hdl.handle.net/10356/96332
http://hdl.handle.net/10220/10164
ISSN: 1059-1524
DOI: 10.1091/mbc.E12-08-0575
Rights: © 2013 Lu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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