Magnetic field enriched surface enhanced resonance Raman spectroscopy for early malaria diagnosis
Date of Issue2012
School of Chemical and Biomedical Engineering
Hemozoin is a by-product of malaria infection in erythrocytes, which has been explored as a biomarker for early malaria diagnosis. We report magnetic field-enriched surface-enhanced resonance Raman spectroscopy (SERRS) of β —hematin crystals, which are the equivalent of hemozoin biocrystals in spectroscopic features, by using magnetic nanoparticles with iron oxide core and silver shell (Fe 3 O 4 @Ag ). The external magnetic field enriches β —hematin crystals and enhances the binding between β —hematin crystals and magnetic nanoparticles, which provides further improvement in SERRS signals. The magnetic field-enriched SERRS signal of β —hematin crystals shows approximately five orders of magnitude enhancement in the resonance Raman signal, in comparison to about three orders of magnitude improvement in the SERRS signal without the influence of magnetic field. The improvement has led to a β —hematin detection limit at a concentration of 5 nM (roughly equivalent to 30 parasites/μl at the early stages of malaria infection), which demonstrates the potential of magnetic field-enriched SERRS technique in early malaria diagnosis.
Journal of biomedical optics
© 2012 Society of Photo-Optical Instrumentation Engineers . This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-Optical Instrumentation Engineers . The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/1.JBO.17.1.017005]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.