Scattering matrix elements of biological particles measured in a flow through system : theory and practice.
Sloot, Peter M. A.
Hoekstra, Alfons G.
Liet, Hans van der.
Figdor, Carl G.
Date of Issue1989
School of Computer Engineering
Light scattering techniques (including depolarization experiments) applied to biological cells provide a fast nondestructive probe that is very sensitive to small morphological differences. Until now quantitative measurement of these scatter phenomena were only described for particles in suspension. In this paper we discuss the symmetry conditions applicable to the scattering matrices of monodisperse biological cells in a flow cytometer and provide evidence that quantitative measurement of the elements of these scattering matrices is possible in flow through systems. Two fundamental extensions to the theoretical description of conventional scattering experiments are introduced: large cone integration of scattering signals and simultaneous implementation of the localization principle to account for scattering by a sharply focused laser beam. In addition, a specific calibration technique is proposed to account for depolarization effects of the highly specialized optics applied in flow through equipment.
DRNTU::Engineering::Computer science and engineering
© 1989 Optical Society of America. This paper was published in Applied Optics and is made available as an electronic reprint (preprint) with permission of Optical Society of America. The paper can be found at the following official DOI: [http://dx.doi.org/10.1364/AO.28.001752]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.