Elastic light scattering from nucleated blood cells : rapid numerical analysis.
Sloot, Peter M. A.
Figdor, Carl G.
Date of Issue1986
School of Computer Engineering
A model is presented to calculate the light-scatter properties of nucleated blood cells which are mimicked by two concentric spheres. The light-scatter characteristics were derived from the Rayleigh-Debye-Gans approximation and by simultaneous application of field averaging of the internal field and modification of the propagation constant inside the different cellular compartments. It is shown that the results obtained with this simple model are similar to those obtained with more complex and more exact theories. In addition it is demonstrated that simultaneous detection of the light-scatter intensities in the forward-, lateral-, and backscatter directions is required to optimize the detection of different cell types in heterogeneous populations of nucleated blood cells.
DRNTU::Engineering::Computer science and engineering
© 1986 Optical Society of America. This paper was published in Applied Optics and is made available as an electronic reprint (preprint) with permission of Optical Society of America. The paper can be found at the following official DOI: [http://dx.doi.org/10.1364/AO.25.003559]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.