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|Title:||Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers||Authors:||Bao, Hongqian
Gan, Leong Huat
|Issue Date:||2012||Source:||Bao, H., Ping, Y., Pan, Y., Li, L., Li, J., & Gan, L. H. (2012). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. Soft Matter, 8(8), 2518-2526.||Series/Report no.:||Soft matter||Abstract:||Serial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5–35.1 °C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h increased 1–4 fold compared to that incubated at 37 °C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of “gold-standard” PEI (25 kDa).||URI:||https://hdl.handle.net/10356/96683
|ISSN:||1744-683X||DOI:||10.1039/c2sm07083a||Rights:||© 2012 The Royal Society of Chemistry.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||MAE Journal Articles|
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