Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/96683
Title: Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers
Authors: Bao, Hongqian
Ping, Yuan
Pan, Yongzheng
Li, Lin
Li, Jun
Gan, Leong Huat
Issue Date: 2012
Source: Bao, H., Ping, Y., Pan, Y., Li, L., Li, J., & Gan, L. H. (2012). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. Soft Matter, 8(8), 2518-2526.
Series/Report no.: Soft matter
Abstract: Serial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5–35.1 °C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h increased 1–4 fold compared to that incubated at 37 °C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of “gold-standard” PEI (25 kDa).
URI: https://hdl.handle.net/10356/96683
http://hdl.handle.net/10220/10348
ISSN: 1744-683X
DOI: http://dx.doi.org/10.1039/c2sm07083a
Rights: © 2012 The Royal Society of Chemistry.
metadata.item.grantfulltext: none
metadata.item.fulltext: No Fulltext
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