Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/95930
Title: Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains
Authors: Liu, Fang
Soh, Annie Yan Ni
Lim, Yingjie
Mohanram, Harini
Bhattacharjya, Surajit
Xing, Bengang
Issue Date: 2012
Source: Liu, F., Soh, A. Y. N., Lim, Y., Mohanram, H., Bhattacharjya, S., & Xing, B. (2012). Lipopolysaccharide Neutralizing Peptide–Porphyrin Conjugates for Effective Photoinactivation and Intracellular Imaging of Gram-Negative Bacteria Strains. Bioconjugate Chemistry, 23(8), 1639-1647.
Series/Report no.: Bioconjugate chemistry
Abstract: A simple and specific strategy based on the bioconjugation of a photosensitizer protophophyrin IX (PpIX) with a lipopolysaccharide (LPS) binding antimicrobial peptide YI13WF (YVLWKRKRKFCFI-Amide) has been developed for the effective fluorescent imaging and photodynamic inactivation of Gram-negative bacterial strains. The intracellular fluorescent imaging and photodynamic antimicrobial chemotherapy (PACT) studies supported our hypothesis that the PpIX-YI13WF conjugates could serve as efficient probes to image the bacterial strains and meanwhile indicated the potent activities against Gram-negative bacterial pathogens especially for those with antibiotics resistance when exposed to the white light irradiation. Compared to the monomeric PpIX-YI13WF conjugate, the dimeric conjugate indicated the stronger fluorescent imaging signals and higher photoinactivation toward the Gram-negative bacterial pathogens throughout the whole concentration range. In addition, the photodynamic bacterial inactivation also demonstrated more potent activity than the minimum inhibitory concentration (MIC) values of dimeric PpIX-YI13WF conjugate itself observed for E. coli DH5a (4 times), S. enterica (8 times), and other Gram-negative strains including antibiotic-resistant E. coli BL21 (8 times) and K. pneumoniae (16 times). Moreover, both fluorescent imaging and photoinactivation measurements also demonstrated that the dimeric PpIX-YI13WF conjugate could selectively recognize bacterial strains over mammalian cells and generate less photo damage to mammalian cells. We believed that the enhanced fluorescence and bacterial inactivation were probably attributed to the higher binding affinity between dimeric photosensitizer peptide conjugate and LPS components on the surface of bacterial strains, which were the results of efficient multivalent interactions.
URI: https://hdl.handle.net/10356/95930
http://hdl.handle.net/10220/10770
ISSN: 1043-1802
DOI: http://dx.doi.org/10.1021/bc300203d
Rights: © 2012 American Chemical Society.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles
SPMS Journal Articles

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.