Red, green, and blue gray-value shift-based approach to whole-field imaging for tissue diagnostics
Krishnan, Mohankumar Valiyambath
Matham, Murukeshan Vadakke
Bhakoo, Kishore K.
Date of Issue2012
School of Mechanical and Aerospace Engineering
Identification of abnormal pathology in situ remains one of the challenges of medicine. The interpretation of tissue conditions relies mainly on optical assessment, which can be difficult due to inadequate visual differences or poor color delineation. We propose a methodology to identify regions of abnormal tissue in a targeted area based on red, green, blue (RGB) shift analysis employing a simple CCD color camera and light-emitting diode illumination in a whole-field-imaging scheme. The concept involves analysis of RGB components in an image with respect to a reference set of RGB values under different illumination wavelengths. The magnitude of the gray value shift is estimated by calculating the Euclidean distance between their normalized RGB coordinates. The shift values obtained using these concepts are thereafter used to construct pseudo-colored images with high contrast, enabling easy identification of abnormal areas in the tissue. Images processed from experiments conducted with excised Wistar rat colon sample (lightly doped with Alexafluor 488) and with simulated tumor (cancer cell pellet placed on colon) showed clear localization of tumor region. This proposed approach and methodology is expected to find potential applications for the in vivo diagnosis of disease.
Journal of biomedical optics
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-Optical Instrumentation Engineers (SPIE). The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/1.JBO.17.7.076010]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.